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Colorimetric loop-mediated isothermal amplification (LAMP) for cost-effective and quantitative detection of SARS-CoV-2: the change in color in LAMP-based assays quantitatively correlates with viral copy number
Analytical Methods ( IF 2.7 ) Pub Date : 2020-12-18 , DOI: 10.1039/d0ay01658f
Everardo González-González 1, 2, 3, 4, 5 , Itzel Montserrat Lara-Mayorga 1, 2, 3, 4, 6 , Iram Pablo Rodríguez-Sánchez 4, 7, 8, 9, 10 , Yu Shrike Zhang 11, 12, 13, 14, 15 , Sergio O. Martínez-Chapa 2, 3, 4, 6 , Grissel Trujillo-de Santiago 1, 2, 3, 4, 6 , Mario Moisés Alvarez 1, 2, 3, 4, 5
Affiliation  

We demonstrate a loop-mediated isothermal amplification (LAMP) method to detect and amplify SARS-CoV-2 genetic sequences using a set of in-house designed initiators that target regions encoding the N protein. We were able to detect and amplify SARS-CoV-2 nucleic acids in the range of 62 to 2 × 105 DNA copies by this straightforward method. Using synthetic SARS-CoV-2 samples and RNA extracts from patients, we demonstrate that colorimetric LAMP is a quantitative method comparable in diagnostic performance to RT-qPCR (i.e., sensitivity of 92.85% and specificity of 81.25% in a set of 44 RNA extracts from patients analyzed in a hospital setting).

中文翻译:

比色环介导的等温扩增(LAMP)用于经济有效地定量检测SARS-CoV-2:基于LAMP的测定中颜色的变化与病毒拷贝数定量相关

我们展示了一种环介导的等温扩增(LAMP)方法,以使用针对目标编码N蛋白的内部设计的一组启动子来检测和扩增SARS-CoV-2遗传序列。通过这种简单的方法,我们能够检测和扩增62至2×10 5个DNA拷贝范围内的SARS-CoV-2核酸。使用合成的SARS-CoV-2样本和患者的RNA提取物,我们证明比色LAMP是一种定量方法,其诊断性能可与RT-qPCR媲美(,在44种RNA提取物中,灵敏度为92.85%,特异性为81.25%来自在医院环境中分析过的患者)。
更新日期:2021-01-05
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