Colorimetric loop-mediated isothermal amplification (LAMP) for cost-effective and quantitative detection of SARS-CoV-2: the change in color in LAMP-based assays quantitatively correlates with viral copy number,Analytical Methods

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Colorimetric loop-mediated isothermal amplification (LAMP) for cost-effective and quantitative detection of SARS-CoV-2: the change in color in LAMP-based assays quantitatively correlates with viral copy number
Analytical Methods ( IF 2.7 ) Pub Date : 2020-12-18 , DOI: 10.1039/d0ay01658f
Everardo González-González _{1,

2,

3,

4,

5} , Itzel Montserrat Lara-Mayorga _{1,

2,

3,

4,

6} , Iram Pablo Rodríguez-Sánchez _{4,

7,

8,

9,

10} , Yu Shrike Zhang _{11,

12,

13,

14,

15} , Sergio O. Martínez-Chapa _{2,

3,

4,

6} , Grissel Trujillo-de Santiago _{1,

2,

3,

4,

6} , Mario Moisés Alvarez _{1,

2,

3,

4,

5}

Affiliation

We demonstrate a loop-mediated isothermal amplification (LAMP) method to detect and amplify SARS-CoV-2 genetic sequences using a set of in-house designed initiators that target regions encoding the N protein. We were able to detect and amplify SARS-CoV-2 nucleic acids in the range of 62 to 2 × 10⁵ DNA copies by this straightforward method. Using synthetic SARS-CoV-2 samples and RNA extracts from patients, we demonstrate that colorimetric LAMP is a quantitative method comparable in diagnostic performance to RT-qPCR (i.e., sensitivity of 92.85% and specificity of 81.25% in a set of 44 RNA extracts from patients analyzed in a hospital setting).

中文翻译：

比色环介导的等温扩增（LAMP）用于经济有效地定量检测SARS-CoV-2：基于LAMP的测定中颜色的变化与病毒拷贝数定量相关

我们展示了一种环介导的等温扩增（LAMP）方法，以使用针对目标编码N蛋白的内部设计的一组启动子来检测和扩增SARS-CoV-2遗传序列。通过这种简单的方法，我们能够检测和扩增62至2×10 ^5个DNA拷贝范围内的SARS-CoV-2核酸。使用合成的SARS-CoV-2样本和患者的RNA提取物，我们证明比色LAMP是一种定量方法，其诊断性能可与RT-qPCR媲美（即，在44种RNA提取物中，灵敏度为92.85％，特异性为81.25％来自在医院环境中分析过的患者）。

更新日期：2021-01-05

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