Majority of the genes expressed during spermatogenesis are autosomal. Mice with different deletions of Yq show sub-fertility, sterility and sperm abnormalities. The connection between Yq deletion and autosomal gene regulation is not well understood. We describe a novel mouse Yq-derived long noncoding RNA, Pirmy, which shows unprecedented number of splice variants in testis. Further, Pirmy transcript variants act as templates for several piRNAs. We identified ten differentially expressed autosome-encoded sperm proteins in mutant mice. Pirmy transcript variants have homology to &5acute/&3acuteUTRs of these deregulated autosomal genes. Thus, subfertility in Y-deleted mice appears to be a polygenic phenomenon that is partially regulated epistatically by the Y-chromosome. Our study provides novel insights into possible role of MSY-derived ncRNAs in male fertility and reproduction. Finally, sperm phenotypes from the Y-deleted mice seem to be similar to that reported in inter-specific male-sterile hybrids. Taken together, this study provides novel insights into possible role of Y-derived ncRNAs in male sterility and speciation.

中文翻译：

---

Y染色体非编码RNA通过piRNA调节小鼠睾丸中的常染色体基因表达

在精子发生过程中表达的大多数基因是常染色体的。Yq缺失不同的小鼠表现出不育，不育和精子异常。Yq缺失与常染色体基因调控之间的联系尚不清楚。我们描述了一种新型的小鼠Yq衍生的长非编码RNA，Pirmy，它在睾丸中显示出前所未有的剪接变体数量。此外，Pirmy转录物变体充当几种piRNA的模板。我们在突变小鼠中鉴定了十种差异表达的常染色体编码的精子蛋白。Pirmy转录本变体与这些失调的常染色体基因的＆5acute /＆3acuteUTR具有同源性。因此，Y缺失小鼠的亚生育力似乎是一种多基因现象，其部分受Y染色体上位调控。我们的研究为MSY来源的ncRNA在男性生育和繁殖中的可能作用提供了新颖的见解。最后，来自Y缺失小鼠的精子表型似乎与种间雄性不育杂种中报道的相似。两者合计，这项研究提供了新的见解，以Y来源的ncRNA在雄性不育和物种形成中的可能作用。