Abstract

Honey bees are important pollinators of wild plants and crops. MicroRNAs (miRNAs) are endogenous regulators of gene expression. In this study, we initially determined that the lethal concentration 50 (LC₅₀) of dinotefuran was 0.773 mg/l. Then, the expression profiles and differentially expressed miRNAs (DE miRNAs) in honey bee brains after 1, 5, and 10 d of treatment with the lethal concentration 10 (LC₁₀) of dinotefuran were explored via deep small-RNA sequencing and bioinformatics. In total, 2, 23, and 27 DE miRNAs were identified after persistent exposure to the LC₁₀ of dinotefuran for 1, 5, and 10 d, respectively. Some abundant miRNAs, such as ame-miR-375-3p, ame-miR-281-5p, ame-miR-3786-3p, ame-miR-10-5p, and ame-miR-6037-3p, were extremely significantly differentially expressed. Enrichment analysis suggested that the candidate target genes of the DE miRNAs are involved in the regulation of biological processes, cellular processes, and behaviors. These results expand our understanding of the regulatory roles of miRNAs in honey bee Apis mellifera (Hymenopptera: Apidae) responses to neonicotinoid insecticides and facilitate further studies on the functions of miRNAs in honey bees.

中文翻译：

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呋虫胺对年轻成年蜜蜂（膜翅目：蜂科）脑 miRNA 表达谱的影响

摘要

蜜蜂是野生植物和农作物的重要传粉者。MicroRNAs (miRNAs) 是基因表达的内源性调节剂。在本研究中，我们最初确定呋虫胺的致死浓度 50 (LC ₅₀ ) 为 0.773 mg/l。然后，通过深度小 RNA 测序和生物信息学探索了用致死浓度 10 (LC ₁₀ ) 的呋虫胺处理 1、5 和 10 天后蜜蜂大脑中的表达谱和差异表达的 miRNA (DE miRNA) 。在持续暴露于 LC ₁₀后，总共鉴定出 2、23 和 27 个 DE miRNA呋虫胺分别作用 1、5 和 10 天。一些丰富的miRNA，如ame-miR-375-3p、ame-miR-281-5p、ame-miR-3786-3p、ame-miR-10-5p和ame-miR-6037-3p，非常显着差异表达。富集分析表明，DE miRNA 的候选靶基因参与了生物过程、细胞过程和行为的调节。这些结果扩大我们的miRNA的调控作用的理解蜜蜂蜜蜂（Hymenopptera：蜜蜂科）新烟碱类杀虫剂的反应和促进对蜜蜂miRNA的功能进一步研究。