The genus Burkholderia sensu lato is composed of a diverse and metabolically versatile group of bacterial species. One characteristic thought to be unique for the genus Burkholderia is the presence of two forms each (with and without 2-hydroxylation) of the membrane lipids phosphatidylethanolamine (PE) and ornithine lipids (OLs). Here, we show that only Burkholderia sensu stricto strains constitutively form OLs, whereas all other analyzed strains belonging to the Burkholderia sensu lato group constitutively form the two forms of PE, but no OLs. We selected two model bacteria to study the function of OL in Burkholderia sensu lato: (1) Burkholderia cenocepacia wild-type which constitutively forms OLs and its mutant deficient in the formation of OLs and (2) Robbsia andropogonis (formerly Burkholderia andropogonis) which does not form OL constitutively, and a derived strain constitutively forming OLs. Both were characterized under free-living conditions and during pathogenic interactions with their respective hosts. The absence of OLs in B. cenocepacia slightly affected bacterial growth under specific abiotic stress conditions such as high temperature and low pH. B. cenocepacia lacking OLs caused lower mortality in Galleria mellonella larvae while R. andropogonis constitutively forming OLs triggers an increased formation of reactive oxygen species immediately after infection of maize leaves, suggesting that OLs can have an important role during the activation of the innate immune response of eukaryotes.

属 伯克霍尔德氏菌sensu lato由多种多样且在代谢方面具有多样性的细菌组成。一种被认为是该属独特的特征伯克霍尔德氏菌膜脂磷脂酰乙醇胺（PE）和鸟氨酸脂（OLs）分别存在两种形式（有和没有2-羟基化）。在这里，我们只显示伯克霍尔德氏菌 sensu stricto菌株组成型形成OL，而所有其他分析菌株均属于OL。 伯克霍尔德氏菌sensu lato基团构成PE的两种形式，但没有OL。我们选择了两种模型细菌来研究OL在动物体内的功能伯克霍尔德氏菌 森苏拉托（1） 伯克霍尔德菌 组成型OL的野生型及其缺乏OL形成的突变体和（2） 罗汉果 （以前 拟人伯克霍尔德氏菌）不是组成型地形成OL，而衍生的菌株组成型地形成OL。两者均在自由生活条件下以及与它们各自宿主的致病性相互作用中进行了表征。缺少OL洋葱头孢菌 在特定的非生物胁迫条件下（例如高温和低pH），对细菌的生长影响很小。 洋葱头孢菌 缺乏OLs导致较低的死亡率 梅洛内拉广场 幼虫 罗非鱼 组成型形成的OLs感染玉米叶后立即触发了活性氧物种形成的增加，这表明OLs在激活真核生物的先天免疫应答过程中可以发挥重要作用。