Lipase-catalyzed reactions offer many advantages among which a high degree of selectivity combined with the possibility to convert even non-natural substrates are of particular interest. A major drawback in the applicability of lipases in the conversion of synthetically interesting, non-natural substrates is the substantial insolubility of such substrates in water. The conversion of substrates, natural or non-natural, by lipases generally involves the presence of a water–oil interface. In the present paper, we exploit the fact that the presence of lipases, in particular the lipase from Candida antarctica B (CalB), changes the bending elastic properties of a surfactant monolayer in a bicontinuous microemulsion consisting of D₂O/NaCl -n-(d)-octane-pentaethylene glycol monodecyl ether (C₁₀E₅) in a similar manner as previously observed for amphiphilic block-copolymers. To determine the bending elastic constant, we have used two approaches, small angle neutron scattering (SANS) and neutron spin echo (NSE) spectroscopy. The time-averaged structure from SANS showed a slight decrease in bending elasticity, while on nanosecond time scales as probed with NSE, a stiffening has been observed, which was attributed to adsorption/desorption mechanisms of CalB at the surfactant monolayer. The results allow to derive further information on the influence of CalB on the composition and bending elasticity of the surfactant monolayer itself as well as the underlying adsorption/desorption mechanism.

脂肪酶催化的反应具有许多优点，其中特别关注高度选择性和甚至转化非天然底物的可能性。脂肪酶在合成有趣的非天然底物转化中的适用性的主要缺点是这种底物在水中的基本不溶性。脂肪酶对天然或非天然底物的转化通常涉及水-油界面的存在。在本文中，我们利用脂肪酶的存在这一事实，特别是来自南极假丝酵母B（CalB），改变由D ₂ O / NaCl -n-（d）-辛烷-五乙二醇单癸醚（C ₁₀ E ₅）组成的双连续微乳液中表面活性剂单层的弯曲弹性）的方式与先前对两亲嵌段共聚物所观察到的方式类似。为了确定弯曲弹性常数，我们使用了两种方法，小角度中子散射（SANS）和中子自旋回波（NSE）光谱。SANS的时间平均结构显示弯曲弹性略有降低，而在NSE探测的纳秒级时标中，观察到了变硬，这归因于CalB在表面活性剂单层的吸附/解吸机理。结果允许获得关于CalB对表面活性剂单层本身的组成和弯曲弹性的影响以及潜在的吸附/解吸机理的进一步信息。