Ovine models of pregnancy have been used extensively to study maternal–fetal interactions and have provided considerable insight into nutrient transfer to the fetus. Ovine models have also been utilized to study congenital heart diseases. In this work, we demonstrate a comprehensive assessment of heart function and metabolism using a perinatal model of heart function with the addition of a [U-¹³C]glucose as tracer to study central energy metabolism. Using nuclear magnetic resonance spectroscopy, and metabolic modelling, we estimate myocardial citric acid cycle turnover (normalized for oxygen consumption), substrate selection, and anaplerotic fluxes. This methodology can be applied to studying acute and chronic effects of hormonal signaling in future studies.

中文翻译：

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应用碳 13 同位素分析评估绵羊围产期心肌葡萄糖代谢


绵羊妊娠模型已被广泛用于研究母胎相互作用，并为营养物质向胎儿的转移提供了相当多的见解。绵羊模型也被用来研究先天性心脏病。在这项工作中，我们展示了使用围产期心脏功能模型并添加[U- ¹³ C]葡萄糖作为示踪剂来研究中枢能量代谢，对心脏功能和代谢进行全面评估。使用核磁共振波谱和代谢模型，我们估计心肌柠檬酸循环周转（根据耗氧量标准化）、底物选择和回补通量。这种方法可用于在未来的研究中研究激素信号传导的急性和慢性影响。