Abstract

Chickpea (Cicer arietinum L.) is an important food legume worldwide, providing a source of high-quality protein. Fifteen chickpea samples showing leaf yellowing and root rot were obtained from southern Alberta, Canada, in 2018. Twenty single-spore Fusarium isolates were obtained from those samples. Based on the morphological characteristics, five species of Fusarium were identified, with F. redolens as the predominant species (11 isolates), followed by F. culmorum (3), F. sporotrichioides (3), F. oxysporum (2) and F. equiseti (1). The identification of each isolate was confirmed using partial sequences of the translation elongation factor 1-alpha (EF-1α) gene. Each EF-1α sequence displayed 99.54% to 100% similarity to sequences for each corresponding species in the GenBank database. The Fusarium isolates were tested for pathogenicity on chickpea ‘CDC Frontier’ in a greenhouse. Isolates recovered from inoculated plants were confirmed as the respective Fusarium species, fulfiling Koch’s postulates. Selected isolates of each Fusarium species were able to infect common crops including barley, corn, canola, and lentil in Alberta. This is the first report of F. redolens on chickpea in Alberta, F. equiseti on chickpea in Canada, and F. culmorum and F. sporotrichioides on chickpea in the world.

摘要

鹰嘴豆（Cicer arietinum L.）是世界范围内重要的食用豆类，提供优质蛋白质的来源。2018 年从加拿大艾伯塔省南部获得了 15 个显示叶黄和根腐病的鹰嘴豆样品。 从这些样品中获得了20 个单孢镰刀菌分离株。基于形态特征，五种的镰孢属进行了鉴定，与F. redolens作为主要物种（11株），其次是F.孢（3），F. sporotrichioides（3），尖孢镰孢（2）和˚F . 木贼(1). 使用翻译延伸因子 1-α ( EF-1α ) 基因的部分序列确认了每个分离株的鉴定。每个EF-1α序列与 GenBank 数据库中每个相应物种的序列显示出 99.54% 到 100% 的相似性。该镰刀菌分离物在温室中对致病性测试的鹰嘴豆“CDC前沿”。从接种植物中回收的分离株被确认为各自的镰刀菌属物种，符合 Koch 的假设。每种镰刀菌属的精选分离株都能感染艾伯塔省的常见作物，包括大麦、玉米、油菜和扁豆。这是艾伯塔省鹰嘴豆上F. redolens的第一份报告，加拿大的鹰嘴豆上有木贼木贼 ( F. equiseti)，世界上鹰嘴豆上的木贼 F. culmorum和F. sporotrichioides。