MiR‐455‐5p monitors myotube morphogenesis by targeting mylip,Journal of Cellular Biochemistry

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MiR‐455‐5p monitors myotube morphogenesis by targeting mylip
Journal of Cellular Biochemistry ( IF 3.0 ) Pub Date : 2021-01-05 , DOI: 10.1002/jcb.29873
Yan-Hua Dong ₁ , Cheng-Jie Zhou ₁ , Meng-Yuan Zhang ₁ , Jin Tao ₁ , Xiao-Meng Zhang ₁ , Lu An ₁ , Ju Zhang ₁ , Jie Yang ₁ , Dong-Jun Liu ₁ , Ming Cang ₁

Affiliation

As a posttranscriptional regulatory factor, microRNA (miRNA) plays an important role in the formation of myotubes. However, little is known about the mechanism of miRNA regulating myotube morphogenesis. Here, we aimed to characterize the function of miR‐455‐5p in myotube morphogenesis by inducing differentiation in C2C12 myoblasts containing murine Mylip fragments with the miR‐455‐5p target sequence. We found that miR‐455‐5p overexpression promoted the differentiation and hypertrophy of myotubes, while miR‐455‐5p inhibition led to the failure of myotube differentiation and formation of short myotubes. Furthermore, we demonstrated that miR‐455‐5p directly targeted the Mylip 3’‐untranslated region, which plays a key role in monitoring myotube morphogenesis. Interestingly, the expression and function of Mylip were opposite to those of miR‐455‐5p during myogenesis. Our data uncovered novel miR‐455‐5p targets and established a functional link between Mylip and myotube morphogenesis. Understanding the involvement of Mylip in myotube morphogenesis provides insight into the function of the gene regulatory network.

中文翻译：

MiR-455-5p 通过靶向 mylip 监测肌管形态发生

microRNA (miRNA) 作为转录后调控因子，在肌管形成过程中发挥着重要作用。然而，关于miRNA调节肌管形态发生的机制知之甚少。在这里，我们旨在通过诱导含有具有 miR-455-5p 靶序列的鼠Mylip片段的C2C12 成肌细胞分化来表征 miR-455-5p 在肌管形态发生中的功能。我们发现miR-455-5p过表达促进了肌管的分化和肥大，而miR-455-5p抑制导致肌管分化失败和短肌管形成。此外，我们证明 miR-455-5p 直接靶向Mylip3'-非翻译区，在监测肌管形态发生中起关键作用。有趣的是，在肌生成过程中，Mylip的表达和功能与 miR-455-5p的表达和功能相反。我们的数据发现了新的 miR-455-5p 靶点，并在Mylip和肌管形态发生之间建立了功能联系。了解Mylip在肌管形态发生中的参与可以深入了解基因调控网络的功能。

更新日期：2021-02-16

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