Antimicrobial, cytotoxic, antioxidants, enzyme inhibition activities, and scanning electron microscopy of Lactuca orientalis (Boiss.) Boiss. seeds,Microscopy Research and Technique

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Antimicrobial, cytotoxic, antioxidants, enzyme inhibition activities, and scanning electron microscopy of Lactuca orientalis (Boiss.) Boiss. seeds
Microscopy Research and Technique ( IF 2.0 ) Pub Date : 2021-01-05 , DOI: 10.1002/jemt.23687
Syeda Anber Zahra ₁ , Javed Iqbal _{1,

2} , Banzeer Ahsan Abbasi ₁ , Amir Shahbaz ₁ , Sobia Kanwal ₃ , Syed Lal Shah ₄ , Parvaiz Ahmad _{5,

6} , Tariq Mahmood ₁

Affiliation

Lactuca orientalis (Boiss.) Boiss. is one of the most frequently used ethnomedicinal plant. This research study was designed to decipher the phytochemical screening, pharmacological potential and implementation of scanning electron microscope (SEM). Six different solvents were used to prepare L. orientalis (LO) seed extracts. Phytochemical and antioxidant activities were determined calorimetrically. To investigate antidiabetic, α-amylase inhibition assay was performed. Brine shrimp assay was performed for cytotoxicity and anti-leishmanial via MTT assay. Disc-diffusion assay was performed to detect protein kinase inhibitory, antibacterial and antifungal activities. SEM was used as identification tool. Significant amount of phenolic and flavonoid content were identified in methanol extract (LOSM) (95.76 ± 3.71 GAE/mg) and (77 ± 3.60 QE/mg). Highest DPPH scavenging potential (82%) was reported for LOSM. Significant total antioxidant capacity (90.60 ± 1.55 AAE/mg) and total reducing power (94.44 ± 1.38 AAE/mg) were determined for LOSM. Highest α-amylase inhibition was found in LOSM (78.20 ± 1.58%). The highest LD₅₀ of brine shrimp was found for n-Hexane extract (LOSH) 13.03 𝜇g/ml. All extracts showed strong anti-leishmanial activity except LOSH. L. orientalis seeds showed significant protein kinase inhibition, antibacterial and antifungal activities. The seeds of L. orientalis were seen to be oblong to obovate, projections, wavy slightly straight, anticlinal wall was raised with apex acuminate. The outer-periclinal wall convex with fine texture. In conclusion, our findings scientifically support ethnomedicinal and biological potentials of L. orientalis seeds. In future, L. orientalis seeds need to be explored for identification and isolation of bioactive compounds. The results obtained necessitate further in vivo studies to evaluate their pharmacological potentials.

中文翻译：

Lactuca orientalis (Boiss.) Boiss 的抗菌、细胞毒性、抗氧化剂、酶抑制活性和扫描电子显微镜。种子

Lactuca orientalis (Boiss.) Boiss。是最常用的民族药用植物之一。本研究旨在解读扫描电子显微镜 (SEM) 的植物化学筛选、药理学潜力和实施。使用六种不同的溶剂制备东方乳杆菌(LO) 种子提取物。植物化学和抗氧化活性通过量热法测定。为了研究抗糖尿病药，进行了α-淀粉酶抑制试验。通过 MTT 试验对盐水虾进行细胞毒性和抗利什曼原虫试验。进行圆盘扩散试验以检测蛋白激酶抑制、抗菌和抗真菌活性。SEM被用作识别工具。在甲醇提取物 (LOSM) (95.76 ± 3.71 GAE/mg) 和 (77 ± 3.60 QE/mg) 中鉴定出大量酚类和类黄酮含量。据报道，LOSM 具有最高的 DPPH 清除潜力 (82%)。对 LOSM 测定了显着的总抗氧化能力 (90.60 ± 1.55 AAE/mg) 和总还原能力 (94.44 ± 1.38 AAE/mg)。在 LOSM 中发现了最高的 α-淀粉酶抑制 (78.20 ± 1.58%)。最高LD ₅₀发现丰年虾的正己烷提取物 (LOSH) 为 13.03 𝜇g/ml。除 LOSH 外，所有提取物均显示出强烈的抗利什曼原虫活性。L. orientalis种子显示出显着的蛋白激酶抑制、抗菌和抗真菌活性。L. orientalis的种子长圆形至倒卵形，突起，波浪状微直，背斜壁凸起，先端渐尖。外周壁凸出，质地细腻。总之，我们的研究结果在科学上支持东方乳杆菌种子的民族医学和生物学潜力。将来，L. orientalis需要探索种子以鉴定和分离生物活性化合物。获得的结果需要进一步的体内研究来评估它们的药理学潜力。

更新日期：2021-01-05

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