Bcl2 family proteins play a critical role in cell death or survival. BAX, the death‐promoting protein of bcl2 family, mediated mitochondrial pathway inducing cells’ apoptosis in mammal. MiRNAs have been implicated as negative regulators down‐regulating genes’ expression after post‐transcriptional level. At present, little is known about the regulatory mechanism of miRNA on the Bcl2 family proteins during CyHV‐2 infection in silver crucian carp (Carassius auratus gibelio). In this study, the ccBAX (silver crucian carp BAX) gene was cloned and expressed, and polyclonal antibodies were raised in mouse against the purified ccBAX‐GST fusion protein. The structure analysis indicated that ccBAX protein included four conserve domains (BH1, BH2, BH3 and transmembrane domains) and the expression of ccBAX protein occurred throughout the cells. Furthermore, two miRNAs (miR‐124 and miRNA‐29b) were identified to negatively regulate ccBAX gene expression in GiCF cell. miR‐124 was found to suppress the expression of WT‐ccBAX (wild type), but not the MT‐ccBAX (mutant). Overall, the results demonstrated that the expression of the ccBAX gene was significantly down‐regulated by miR‐124 in silver crucian carp (Carassius auratus gibelio) during CyHV‐2 infection.

中文翻译：

---

塞浦路斯疱疹病毒2型感染后，miR-124在金龟子尾鳍细胞中表达和调节ccBAX

Bcl2家族蛋白在细胞死亡或存活中起关键作用。BAX是bcl2家族的促死亡蛋白，它通过介导的线粒体途径诱导哺乳动物细胞凋亡。在转录后水平后，miRNA被认为是负调节剂，下调基因的表达。目前，很少CyHV-2感染期间在银鲫（已知大约miRNA的上Bcl2家族蛋白的调节机制银鲫）。在这项研究中，克隆并表达了ccBAX（银cru鱼BAX）基因，并在小鼠中产生了针对纯化的ccBAX-GST融合蛋白的多克隆抗体。结构分析表明，ccBAX蛋白包括四个保守结构域（BH1，BH2，BH3和跨膜结构域），并且ccBAX蛋白的表达遍及整个细胞。此外，还鉴定出两个miRNA（miR-124和miRNA-29b）对GiCF细胞中的ccBAX基因表达负调控。发现miR-124抑制WT-ccBAX（野生型）的表达，但不抑制MT-ccBAX（突变型）的表达。总体而言，结果表明在CyHV-2感染期间，miR-124在silver鱼（Carassius auratus gibelio）中的ccBAX基因表达显着下调。