Temperature regulation as a tool to program synthetic microbial community composition,Biotechnology and Bioengineering

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Temperature regulation as a tool to program synthetic microbial community composition
Biotechnology and Bioengineering ( IF 3.5 ) Pub Date : 2021-01-05 , DOI: 10.1002/bit.27662
Adam G Krieger ₁ , Jiahao Zhang ₂ , Xiaoxia N Lin _{2,

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Affiliation

Engineering of synthetic microbial communities is emerging as a powerful new paradigm for performing various industrially, medically, and environmentally important processes. To reach the fullest potential, however, this approach requires further development in many aspects, a key one being regulating the community composition. Here we leverage well‐established mechanisms in ecology which govern the relative abundance of multispecies ecosystems and develop a new tool for programming the composition of synthetic microbial communities. Using a simple model system consisting of two microorganisms Escherichia coli and Pseudomonas putida, which occupy different but partially overlapping thermal niches, we demonstrated that temperature regulation could be used to enable coexistence and program the community composition. We first investigated a constant temperature regime and showed that different temperatures led to different community compositions. Next, we invented a new cycling temperature regime and showed that it can dynamically tune the microbial community, achieving a wide range of compositions depending on parameters that are readily manipulatable. Our work provides conclusive proof of concept that temperature regulation is a versatile and powerful tool capable of programming compositions of synthetic microbial communities.

中文翻译：

温度调节作为编程合成微生物群落组成的工具

合成微生物群落工程正在成为一种强大的新范式，用于执行各种工业、医学和环境重要过程。然而，为了充分发挥潜力，这种方法需要在许多方面进一步发展，其中一个关键是调节群落组成。在这里，我们利用了管理多物种生态系统相对丰度的生态学中成熟的机制，并开发了一种用于编程合成微生物群落组成的新工具。使用由两种微生物大肠杆菌和恶臭假单胞菌组成的简单模型系统，它们占据不同但部分重叠的热生态位，我们证明了温度调节可用于实现共存和规划群落组成。我们首先研究了恒温状态，并表明不同的温度会导致不同的群落组成。接下来，我们发明了一种新的循环温度方案，并表明它可以动态调整微生物群落，根据易于操作的参数实现广泛的组成。我们的工作提供了确凿的概念证明，即温度调节是一种通用且强大的工具，能够对合成微生物群落的组成进行编程。

更新日期：2021-02-18

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