Environmental Technology & Innovation ( IF 6.7 ) Pub Date : 2021-01-05 , DOI: 10.1016/j.eti.2021.101356 Ganesh Swain , R.K. Sonwani , R.S. Singh , Ravi P. Jaiswal , B.N. Rai
In this work, Bacillus flexus GS1 IIT (BHU) as free and immobilized cells on low-density polyethylene (LDPE) were used to remove 4-Chlorophenol (4-CP) from the synthetic wastewater. The effect of the process variables, namely process time, pH, temperature, and 4-CP concentration, were optimized and obtained as 7.0 days, 7.0, 30 °C, and 50 mg/L, respectively. At optimum conditions, the maximum removal efficiencies (REs) were obtained to be 90.34 and 78.12% for the immobilized and free cells, respectively, at 50 mg/L concentration of 4-CP. Furthermore, a packed bed bioreactor (PBBR) was continuously operated for 60 days with the immobilized LDPE. The maximum RE of 82.4% and elimination capacity (EC) of 29.6 mg/L/d were obtained at 50 mg/L of the initial concentration of 4-CP. The growth kinetic parameters for Bacillus sp. were obtained to be: : 0.205 h−1, : 3.3 mg/L, : 220 mg/L for Aiba model; : 0.197 h−1, : 1.4 mg/L, : 220 mg/L for Edward model; and : 0.185 h−1, : 1.6 mg/L, : 200 mg/L for Andrew-Haldane model, respectively.
中文翻译:
游离芽孢杆菌作为游离固定体系去除4-氯苯酚:过程变量和动力学研究的影响
在这项工作中,使用弯曲杆菌芽孢杆菌GS1 IIT(BHU)作为游离细胞并将其固定在低密度聚乙烯(LDPE)上,以从合成废水中去除4-氯苯酚(4-CP)。优化了工艺变量的影响,即工艺时间,pH,温度和4-CP浓度,分别为7.0天,7.0、30°C和50 mg / L。在最佳条件下,浓度为50 mg / L的4-CP时,固定细胞和游离细胞的最大去除效率(REs)分别为90.34%和78.12%。此外,填充床生物反应器(PBBR)与固定的LDPE连续运行60天。在4-CP的初始浓度下,获得50mg / L的最大RE和89.6%的消除容量(EC)。的生长动力学参数芽孢杆菌属。获得为::0.205 h -1,:3.3毫克/升, :Aiba模型为220 mg / L; :0.197 h -1,:1.4毫克/升 :Edward型号220 mg / L; 和:0.185 h -1,:1.6毫克/升, :Andrew-Haldane模型分别为200 mg / L。