In this work, Bacillus flexus GS1 IIT (BHU) as free and immobilized cells on low-density polyethylene (LDPE) were used to remove 4-Chlorophenol (4-CP) from the synthetic wastewater. The effect of the process variables, namely process time, pH, temperature, and 4-CP concentration, were optimized and obtained as 7.0 days, 7.0, 30 °C, and 50 mg/L, respectively. At optimum conditions, the maximum removal efficiencies (REs) were obtained to be 90.34 and 78.12% for the immobilized and free cells, respectively, at 50 mg/L concentration of 4-CP. Furthermore, a packed bed bioreactor (PBBR) was continuously operated for 60 days with the immobilized LDPE. The maximum RE of 82.4% and elimination capacity (EC) of 29.6 mg/L/d were obtained at 50 mg/L of the initial concentration of 4-CP. The growth kinetic parameters for Bacillus sp. were obtained to be: ${\mu }_{max}$: 0.205 h⁻¹, ${\mathit{K}}_s$: 3.3 mg/L, ${\mathit{K}}_i$: 220 mg/L for Aiba model; ${\mu }_{max}$: 0.197 h⁻¹, ${\mathit{K}}_s$: 1.4 mg/L, ${\mathit{K}}_i$: 220 mg/L for Edward model; and $m{u}_{max}$: 0.185 h⁻¹, ${\mathit{K}}_s$: 1.6 mg/L, ${\mathit{K}}_i$: 200 mg/L for Andrew-Haldane model, respectively.

在这项工作中，使用弯曲杆菌芽孢杆菌GS1 IIT（BHU）作为游离细胞并将其固定在低密度聚乙烯（LDPE）上，以从合成废水中去除4-氯苯酚（4-CP）。优化了工艺变量的影响，即工艺时间，pH，温度和4-CP浓度，分别为7.0天，7.0、30°C和50 mg / L。在最佳条件下，浓度为50 mg / L的4-CP时，固定细胞和游离细胞的最大去除效率（REs）分别为90.34％和78.12％。此外，填充床生物反应器（PBBR）与固定的LDPE连续运行60天。在4-CP的初始浓度下，获得50mg / L的最大RE和89.6％的消除容量（EC）。的生长动力学参数芽孢杆菌属。获得为：${\mu }_{米\mathrm{一种}X}$：0.205 h ^-1，${\mathit{ķ}}_s$：3.3毫克/升， ${\mathit{ķ}}_{\mathrm{一世}}$：Aiba模型为220 mg / L; ${\mu }_{米\mathrm{一种}X}$：0.197 h ^-1，${\mathit{ķ}}_s$：1.4毫克/升 ${\mathit{ķ}}_{\mathrm{一世}}$：Edward型号220 mg / L; 和$米{ü}_{米\mathrm{一种}X}$：0.185 h ^-1，${\mathit{ķ}}_s$：1.6毫克/升， ${\mathit{ķ}}_{\mathrm{一世}}$：Andrew-Haldane模型分别为200 mg / L。