The Sugarcane white leaf (SCWL) phytoplasma from group 16SrXI is the causal agent of Sugarcane white leaf disease (SWLD) resulting in severe yield losses to the sugar industry. Although microscopy, serology based methods, dot blot DNA hybridization, and PCR have been developed as diagnostic tests, these techniques are labour intensive, costly and time-consuming and can only be undertaken in well-equipped laboratories. Therefore, the objective of the present study was to develop a loop-mediated isothermal amplification (LAMP) assay for SCWL as an alternative approach for quick and efficient detection of the sugarcane white leaf (SCWL) phytoplasma within 30 minutes. Three LAMP primer sets including a universal 16S rRNA based phytoplasma assay and two 16SrXI group-specific assays were used to detect the 16SrXI SCWL phytoplasma. Plant cytochrome oxidase (cox) LAMP primers that amplify a housekeeping gene in plants were used as controls. LAMP assays using a commercially available master mix and a real-time fluorometer allowed detection of the SCWL phytoplasma within 30 minutes at 63^oC via the visualization of an amplification plot on screen. The results obtained in this study revealed potential applicability of the LAMP-based assay with 16SrXI SCWL primers for in-field detection of SCWL in comparison with traditional methods that are labour-intensive and prone to cross-contamination during their implementation

16SrXI组的甘蔗白叶（SCWL）植物质原体是甘蔗白叶病（SWLD）的病原体，导致制糖业严重损失产量。尽管已经开发了显微镜，基于血清学的方法，斑点印迹DNA杂交和PCR作为诊断测试方法，但这些技术是劳动密集型，昂贵且费时的，并且只能在设备完善的实验室中进行。因此，本研究的目的是为SCWL开发一种环介导的等温扩增（LAMP）分析，作为在30分钟内快速有效检测甘蔗白叶（SCWL）植物质体的另一种方法。使用三个LAMP引物组（包括基于通用16S rRNA的植物质检测）和两个16SrXI组特异性检测来检测16SrXI SCWL植物质。将在植物中扩增管家基因的植物细胞色素氧化酶（cox）LAMP引物用作对照。使用市售的预混液和实时荧光计进行的LAMP分析可在63分钟的30分钟内检测到SCWL植物质浆^ø通过在屏幕上的扩增曲线的可视化下进行。这项研究中获得的结果表明，与传统的劳动密集型方法在实施过程中容易发生交叉污染的方法相比，使用16SrXI SCWL引物进行的基于LAMP的测定可用于SCWL的现场检测