The screening and identification of bioactive components, which are effectively resistant to metallo-beta-lactamase (MβL), were studied in the alcohol extract of Schisandra chinensis (Turcz.) Baill. by metalloenzyme-immobilized affinity chromatography. Taking bizinc metalloenzyme beta-lactamase II from Bacillus cereus (Bc II) and monozinc metalloenzyme CphA from aeromonas hydrophila (CphA) as examples, we studied the feasibility of this scheme based on the construction of metalloenzyme-immobilized chromatographic model. It was found that the Bc II- and CphA-immobilized chromatographic column could be used not only to explore the interaction between the MβL and their specific ligands, but also to screen the bioactive components from traditional Chinese medicine. The Bc II-and CphA-immobilized columns were used to screen the bioactive components from the alcohol extract of Schisandra chinensis (Turcz.) Baill. Time-of-flight tandem mass spectrometry analysis and molecular docking revealed that isobutyl 3-O-sulfo-β-D-galactopyranoside is the effective bioactive components that could bind with metalloenzyme Bc II. It is believed that our current work may provide a methodological reference for screening MβL inhibitors from traditional Chinese medicine.

在五味子酒（Turcz。）Baill的醇提取物中研究了对金属β-内酰胺酶（MβL）具有有效抗性的生物活性成分的筛选和鉴定。通过金属酶固定的亲和层析。取蜡样芽胞杆菌（Bc II）的bizinc金属酶β-内酰胺酶II和嗜水气单胞菌的单锌金属酶CphA（CphA）为例，我们基于固定化金属酶的色谱模型研究了该方案的可行性。研究发现，固定有Bc II和CphA的色谱柱不仅可用于探索MβL及其特异性配体之间的相互作用，而且还可用于从中药中筛选生物活性成分。Bc II和CphA固定柱用于从五味子的乙醇提取物中筛选生物活性成分（Turcz。）Baill。飞行时间串联质谱分析和分子对接发现，异丁基3-O-磺基-β-D-吡喃半乳糖苷是可以与金属酶Bc II结合的有效生物活性成分。相信我们目前的工作可能为从中药中筛选MβL抑制剂提供方法学参考。