In this study, a versatile process for the production of xylooligosaccharides (XOS) with a low degree of polymerization (DP 2–6) from hazelnut shells was designed. This process included autohydrolysis integrated with sequential enzymatic hydrolysis by crude xylanase produced with Aureobasidium pullulans NRRL Y–2311–1 from wheat bran. Autohydrolysis of hazelnut shells was carried out at a solid:liquid ratio of 1:6 (w/w) and 190 °C non-isothermally. The effects of several parameters on enzymatic hydrolysis of the autohydrolysis liquor were determined. The maximum XOS (DP 2–6) production was 22.5 g/L which was obtained at pH 5.0 and 40 °C using enzyme concentration of 240 U/g XOS and substrate concentration of 72 g/L. Under these conditions, 31.29 % of the substrate (total XOS) was converted to low-DP-XOS; xylobiose and xylotriose are being the major oligomers. This is the first study on the application of A. pullulans xylanase in production of xylooligomers from hazelnut shells.

在这项研究中，设计了一种从榛子壳中生产低聚合度（DP 2-6）的低聚木寡糖（XOS）的通用方法。该过程包括自动水解与顺序酶促水解相结合，并由金黄色葡萄球菌产生的粗木聚糖酶进行酶促水解来自麦麸的NRRL Y-2311-1。榛子壳的自动水解是在非等温下以固液比为1：6（w / w）和190°C进行的。确定了几个参数对自水解液的酶水解的影响。XOS（DP 2-6）的最大产量为22.5 g / L，这是在pH 5.0和40°C下使用240 U / g XOS的酶浓度和72 g / L的底物浓度获得的。在这些条件下，31.29％的底物（总XOS）被转化为低DP-XOS。木二糖和木三糖是主要的低聚物。这是对A的应用的首次研究。从榛子壳中生产木寡聚体中的支链淀粉木聚糖酶。