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Identification of protein/mRNA network involving the PSORS1 locus gene CCHCR1 and the PSORS4 locus gene HAX1
Experimental Cell Research ( IF 3.3 ) Pub Date : 2021-01-05 , DOI: 10.1016/j.yexcr.2021.112471
Cinzia Pisani , Annalisa Onori , Francesca Gabanella , Maria Grazia Di Certo , Claudio Passananti , Nicoletta Corbi

CCHCR1 (Coiled-Coil alpha-Helical Rod 1), maps to chromosomal region 6p21.3, within the major psoriasis susceptibility locus PSORS1. CCHCR1 itself is a plausible psoriasis candidate gene, however its role in psoriasis pathogenesis remains unclear. We previously demonstrated that CCHCR1 protein acts as a cytoplasmic docking site for RNA polymerase II core subunit 3 (RPB3) in cycling cells, suggesting a role for CCHCR1 in vesicular trafficking between cellular compartments.

Here, we report a novel interaction between CCHCR1 and the RNA binding protein HAX1. HAX1 maps to chromosomal region 1q21.3 within the PSORS4 locus and is over-expressed in psoriasis. Both CCHCR1 and HAX1 share subcellular co-localization with mitochondria, nuclei and cytoplasmic vesicles as P-bodies. By a series of ribonucleoprotein immunoprecipitation (RIP) assays, we isolated a pool of mRNAs complexed with HAX1 and/or CCHCR1 proteins. Among the mRNAs complexed with both CCHCR1 and HAX1 proteins, there are Vimentin mRNA, previously described to be bound by HAX1, and CAMP/LL37 mRNA, whose gene product is over-expressed in psoriasis.



中文翻译:

鉴定涉及PSORS1基因座基因CCHCR1和PSORS4基因座基因HAX1的蛋白质/ mRNA网络,鉴定涉及PSORS1基因座基因CCHCR1和PSORS4基因座基因HAX1的蛋白质/ mRNA网络,鉴定涉及PSORS1基因座基因CCHCR1和PSORS4基因座基因HAX1的蛋白质/ mRNA网络

CCHCR1(卷曲螺旋α螺旋杆1)映射到主要牛皮癣易感基因座PSORS1中的染色体区域6p21.3。CCHCR1本身是一个可能的牛皮癣候选基因,但是其在牛皮癣发病机理中的作用仍不清楚。我们以前证明CCHCR1蛋白在循环细胞中充当RNA聚合酶II核心亚基3(RPB3)的胞质停靠位点,提示CCHCR1在细胞区室之间的囊泡运输中发挥作用。

在这里,我们报告CCHCR1和RNA结合蛋白HAX1之间的新型相互作用。HAX1映射到PSORS4基因座内的染色体区域1q21.3,在牛皮癣中过表达。CCHCR1和HAX1都与作为P体的线粒体,细胞核和细胞质囊泡共享亚细胞共定位。通过一系列的核糖核蛋白免疫沉淀(RIP)分析,我们分离了与HAX1和/或CCHCR1蛋白复合的mRNA池。在与CCHCR1和HAX1蛋白复合的mRNA中,有Vimentin mRNA和CAMP / LL37 mRNA,它们先前在HAX1上被结合,而CAMP / LL37 mRNA在牛皮癣中过表达。

,

CCHCR1(卷曲螺旋α螺旋杆1)映射到主要牛皮癣易感基因座PSORS1中的染色体区域6p21.3。CCHCR1本身是一个可能的牛皮癣候选基因,但是其在牛皮癣发病机理中的作用仍不清楚。我们以前证明CCHCR1蛋白在循环细胞中充当RNA聚合酶II核心亚基3(RPB3)的胞质停靠位点,提示CCHCR1在细胞区室之间的囊泡运输中发挥作用。

在这里,我们报告CCHCR1和RNA结合蛋白HAX1之间的新型相互作用。HAX1映射到PSORS4基因座内的染色体区域1q21.3,在牛皮癣中过表达。CCHCR1和HAX1都与作为P体的线粒体,细胞核和细胞质囊泡共享亚细胞共定位。通过一系列的核糖核蛋白免疫沉淀(RIP)分析,我们分离了与HAX1和/或CCHCR1蛋白复合的mRNA池。在与CCHCR1和HAX1蛋白复合的mRNA中,有Vimentin mRNA和CAMP / LL37 mRNA,它们先前在HAX1上被结合,而CAMP / LL37 mRNA在牛皮癣中过表达。

,

CCHCR1(卷曲螺旋α螺旋杆1)映射到主要牛皮癣易感基因座PSORS1中的染色体区域6p21.3。CCHCR1本身是一个可能的牛皮癣候选基因,但是其在牛皮癣发病机理中的作用仍不清楚。我们以前证明CCHCR1蛋白在循环细胞中充当RNA聚合酶II核心亚基3(RPB3)的胞质停靠位点,提示CCHCR1在细胞区室之间的囊泡运输中发挥作用。

在这里,我们报告CCHCR1和RNA结合蛋白HAX1之间的新型相互作用。HAX1映射到PSORS4基因座内的染色体区域1q21.3,在牛皮癣中过表达。CCHCR1和HAX1都与作为P体的线粒体,细胞核和细胞质囊泡共享亚细胞共定位。通过一系列的核糖核蛋白免疫沉淀(RIP)分析,我们分离了与HAX1和/或CCHCR1蛋白复合的mRNA池。在与CCHCR1和HAX1蛋白复合的mRNA中,有Vimentin mRNA和CAMP / LL37 mRNA,它们先前在HAX1上被结合,而CAMP / LL37 mRNA在牛皮癣中过表达。

更新日期:2021-01-14
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