It is well known that sulfite (SO₃²⁻) plays an indispensable role in various physiological processes. Abnormal levels of SO₃²⁻ can trigger a wide variety of diseases involving respiratory, nervous and cardiovascular systems. Hence, it is necessary to find an efficient approach for detection of SO₃²⁻. In this study, a pyrene derivative, (E)-4-(3-oxo-3-(pyren-1-yl)prop-1-en-1-yl)phenyl acrylate (PPA), was designed and synthesized for monitoring SO₃²⁻. The probe possessed simple synthetic steps, excellent anti-interference ability and specific response to SO₃²⁻ in the presence of other substances. The reaction between PPA and SO₃²⁻ was ascribed to Michael addition and the detection mechanism was confirmed by HRMS spectra analysis and FTIR analysis. Additionally, PPA responded linearly to detect SO₃²⁻ within the rang of 0–100 μM. The limit of detection was calculated as low as 0.17 μM in accordance with the recommendation of IUPAC (CDL =3sb/m). Notably, PPA was further applied in biological imaging in HepG2 cells, which provided a possibility to monitor SO₃²⁻ in vivo.

众所周知，亚硫酸盐（SO ₃ ^2-）在各种生理过程中起着不可或缺的作用。SO ₃ ^{2- 的}异常水平可引发多种疾病，包括呼吸系统、神经系统和心血管系统。因此，有必要找到一种有效的方法来检测 SO ₃ ^2-。在这项研究中，芘衍生物，(E)-4-(3-oxo-3-(pyren-1-yl)prop-1-en-1-yl) 苯基丙烯酸酯 (PPA)，被设计和合成用于监测SO ₃ ^2-。该探针合成步骤简单，抗干扰能力强，在其他物质存在下对SO ₃ ^{2- 有}特异性响应。PPA与SO ₃反应^2-归因于迈克尔加成，检测机制通过 HRMS 光谱分析和 FTIR 分析得到证实。此外，PPA 线性响应以检测0–100 μM 范围内的SO ₃ ^2-。根据 IUPAC 的建议（CDL = 3sb/m）计算检测限低至 0.17 μM。值得注意的是，PPA 进一步应用于 HepG2 细胞的生物成像，这提供了在体内监测 SO ₃ ^2-的可能性。