The genus Justicia (Acanthaceae) is a source of important pharmaceutical compounds. Justicia species are integral parts of several drug preparations in Indian indigenous healthcare system. However, J. beddomei and J. adhatoda are the authentic sources of ‘Vasa’. To authenticate assured therapeutic effect, proper identification at species level is critical. Phytochemical studies were carried out in different parts of seven Justicia species, viz., J. adhatoda, J. beddomei, J. betonica, J. carnea, J. gendarussa, J. santapaui and J. wynaadensis, using chromatographic analysis. Extracts of various parts like root, stem and leaf of the selected species were compared. The various parts showed remarkable differents in their chemical profiles. Comparative high-performance thin-layer chromatography (HPTLC) profile of seven Justicia species, various parts such as root, stem and leaf, have been developed and the same can be used as an analytical tool for the identification and authentication of genuine material and also as a ready reckoner to identify the authenticity of market raw drug samples.

Justicia（棘皮科）属是重要药物化合物的来源。Justicia物种是印度本土医疗系统中几种药物制剂的组成部分。然而，J. beddomei和Ĵ。adhatoda是“ Vasa ”的真实来源。为了验证有保证的治疗效果，在物种级别进行正确识别至关重要。植物化学研究，在七个不同的部位进行了正义的物种，即，J.藏药，J. beddomei，J. betonica，J.吉祥草，J. gendarussa，J. santapaui和J. wynaadensis，使用色谱分析。比较了所选物种的根，茎和叶各部分的提取物。各个部分的化学特征显示出显着差异。已经开发了7种Justicia物种（根，茎和叶的各个部分）的比较高效薄层色谱（HPTLC）配置文件，并且可以用作鉴定和鉴定真实材料的分析工具，并且作为确定市场原始药物样品真实性的现成推算工具。