Abstract

The effects of exogenous recombinant human heat shock protein Hsp70 and hydrogen sulfide donor GYY4137 on the mechanisms of endocytosis of lipopolysaccharide (LPS) by human neuroblastoma cells SH-SY5Y were studied. Hsp70 and GYY4137 have been shown to significantly reduce LPS-induced production of inflammatory mediators by SH-SY5Y cells, including reactive oxygen species, nitric oxide, TNFα, IL-1β, and IL-6. Both the recombinant protein Hsp70 and the hydrogen sulfide donor GYY4137 exhibited significant protective effects; however, the combined use of these agents did not lead to a cumulative effect. It has been shown that pinocytosis, as well as clathrin-, caveolin-, tubulin- and receptor-dependent endocytosis were involved in protecting the cells by both the hydrogen sulfide donor and Hsp70 from LPS-induced production of reactive oxygen species and NO.

中文翻译：

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硫化氢供体GYY4137和HSP70蛋白对脂多糖激活SH-SY5Y细胞的影响

摘要

研究了外源重组人热休克蛋白Hsp70和硫化氢供体GYY4137对人成神经细胞瘤细胞SH-SY5Y内脂多糖（LPS）内吞作用机制的影响。Hsp70和GYY4137已显示可显着减少L-PS诱导的SH-SY5Y细胞炎症介质的产生，包括活性氧，一氧化氮，TNFα，IL-1β和IL-6。重组蛋白Hsp70和硫化氢供体GYY4137均显示出显着的保护作用。但是，这些药物的联合使用并没有产生累积效应。研究表明，胞饮作用以及网格蛋白，小窝蛋白，