Micro-liquid dispensing technology is widely used in the field of in vitro diagnostics. To study the influence of the system factors on the liquid dispensing process, a micro-liquid dispensing system based on a syringe pump is designed in this paper, and a Fluent simulation model is established to simulate the dispensing process. Taking into account the existence of the air column in the simulation model, the simulation and analysis of the influence of the system parameters is carried under different air volume conditions, and the reliability of the simulation results are verified through experiments. The experimental and simulation results show that ideal dispensing can be achieved when the surface tension of reagent is large and its viscosity is small. Moreover, breakages, satellite droplets, and bubbles will appear successively with an increase in the outlet diameter when the inlet velocity is large, while adhesion will occur when the inlet velocity is small. These results will be used to guide the design and control of this system.

微液体分配技术广泛用于体外诊断领域。为了研究系统因素对液体分配过程的影响，本文设计了一种基于注射泵的微液体分配系统，并建立了Fluent仿真模型来模拟分配过程。考虑到仿真模型中存在气柱，在不同风量条件下进行了系统参数影响的仿真分析，并通过实验验证了仿真结果的可靠性。实验和仿真结果表明，当试剂的表面张力大，粘度小时，可以达到理想的点胶效果。此外，破损，卫星飞沫，当入口速度大时，气泡将随着出口直径的增加而连续出现，而当入口速度小时，气泡将发生粘附。这些结果将用于指导该系统的设计和控制。