Simple and low-cost solutions are becoming extremely important for the evolving necessities of biomedical applications. Even though, on-chip sample processing and analysis has been rapidly developing for a wide range of screening and diagnostic protocols, efficient and reliable fluid manipulation in microfluidic platforms still require further developments to be considered portable and accessible for low-resource settings. In this work, we present an extremely simple microfluidic pumping device based on three-dimensional (3D) printing and acoustofluidics. The fabrication of the device only requires 3D-printed adaptors, rectangular glass capillaries, epoxy and a piezoelectric transducer. The pumping mechanism relies on the flexibility and complexity of the acoustic streaming patterns generated inside the capillary. Characterization of the device yields controllable and continuous flow rates suitable for on-chip sample processing and analysis. Overall, a maximum flow rate of ~ 12 μL/min and the control of pumping direction by frequency tuning is achieved. With its versatility and simplicity, this microfluidic pumping device offers a promising solution for portable, affordable and reliable fluid manipulation for on-chip applications.

对于生物医学应用不断发展的需求来说，简单且低成本的解决方案变得极其重要。尽管如此，片上样品处理和分析已经在广泛的筛选和诊断方案中迅速发展，但微流控平台中高效可靠的流体操作仍然需要进一步的开发，以便在资源匮乏的环境下实现便携式和可访问性。在这项工作中，我们提出了一种基于三维（3D）打印和声流控的极其简单的微流体泵送装置。该设备的制造只需要 3D 打印适配器、矩形玻璃毛细管、环氧树脂和压电传感器。泵送机制依赖于毛细管内部产生的声流模式的灵活性和复杂性。该器件的表征可产生适合片上样品处理和分析的可控且连续的流速。总体而言，最大流量约为 12 μL/min，并通过频率调谐控制泵送方向。凭借其多功能性和简单性，这种微流体泵送设备为片上应用的便携式、经济实惠且可靠的流体操纵提供了一种有前途的解决方案。