The genetic identity of Wolbachia endosymbiont in wild-caught Culex quinquefasciatus was determined for the first time in Indonesia. A total of 314 Cx. quinquefasciatus were examined for Wolbachia by PCR assay targeting the Wolbachia surface protein (wsp) gene. The prevalence of Wolbachia infection was detected in 29.94% of Cx. specimens (45.86% female and 8.27% male). The group-specific infection was detected with an infection rate of 0.32%, 28.98%, and 0.64% in groups A, B, and A&B, respectively. Phylogenetic analysis revealed all Wolbachia strains from Indonesia were genetically affiliated to the supergroup A and B with the high sequence similarity of 97.9–100% and 99.7–100%, respectively. Phylogenetic relationships can be easily distinguished by neighbor-joining analysis and were congruent by maximum likelihood method. The genetic distance (GD) values of intra- and inter-group analysis indicated a lower level (GD < 0.007 for group A and GD < 0.003 for group B) within the Indonesia strains and a higher level (GD > 1.125 for group A and GD > 1.129 for group B) as compared with other Wolbachia strains. Our results provide the first genetic identification of Wolbachia endosymbiont in Cx. quinquefasciatus collected from Indonesia, and the phylogenetic analysis revealed a new discovery of group A Wolbachia in wild-caught Cx. quinquefasciatus mosquitoes.

在印度尼西亚首次确定了野生捕捞的库蚊中Wolbachia内共生体的遗传特性。总共 314 个Cx。倦检查用于沃尔巴克氏体测定靶向通过PCR沃尔巴克氏体表面蛋白（WSP）基因。在 29.94% 的Cx 中检测到Wolbachia感染的流行。标本（45.86% 女性和 8.27% 男性）。A、B、A&B组感染率分别为0.32%、28.98%和0.64%。系统发育分析揭示了所有Wolbachia来自印度尼西亚的菌株在遗传上属于超群 A 和 B，分别具有 97.9-100% 和 99.7-100% 的高序列相似性。系统发育关系可以通过邻接分析很容易区分，并且通过最大似然方法是一致的。组内和组间分析的遗传距离 (GD) 值表明印度尼西亚菌株内的水平较低（A 组的 GD < 0.007 和 B 组的 GD < 0.003）和较高的水平（A 组和组的 GD > 1.125）。与其他Wolbachia菌株相比，B 组的 GD > 1.129 。我们的结果提供了Cx中Wolbachia内共生体的首次遗传鉴定。从印度尼西亚采集的quinquefasciatus，系统发育分析揭示了A群的新发现野生捕获的Cx 中的Wolbachia 。quinquefasciatus蚊子。