Purpose

To determine whether the blastocyst mitochondrial DNA (mtDNA) content is related to the miscarriage rate in patients undergoing single euploid frozen embryo transfer (SEFET).

Methods

A total of 355 single euploid frozen embryo transfer cycles were studied retrospectively between April 2017 and December 2018. A trophectoderm biopsy was performed on day 5/6 blastocysts. Post next-generation sequencing (NGS), the mtDNA content was calculated as the ratio of mitochondrial DNA over nuclear DNA, and the association between blastocyst mtDNA content and miscarriage rate was evaluated.

Result(s)

Three hundred fifty-five euploid blastocysts were selected for SEFET in 314 patients with an average age of 33.7 ± 5.6 years; 255 were biopsied on day 5 (71.8%) and 100 on day 6 (28.2%). Frozen embryo transfer (FET) was performed either in a hormone replacement therapy (HRT) cycle (71.8%; n = 255) or in a natural cycle (NC) (28.2%; n = 100). A pregnancy rate of 66.2% (235/355) was obtained with clinical pregnancy and miscarriage rates of 52.4% (n = 186) and 5.6% (n = 20), respectively. There was no significant difference neither between the blastocyst mtDNA content of pregnant and nonpregnant patients (27.7 ± 9.2 vs. 29.4 ± 8.6, P = 0.095) nor between patients with a clinical pregnancy and miscarriage (30.5 ± 9.3 vs. 27.3 ± 9.2, P = 0.136). Multivariate logistic regression analysis showed the same nonsignificant relationship, except for the miscarriage rate and BMI (OR 1.149, 95% CI 1.03–1.28; P = 0.012).

Conclusion(s)

Mitochondrial DNA content is unable to predict the miscarriage of implanted human euploid blastocysts.

中文翻译：

---

囊胚线粒体 DNA 含量是否会影响接受单倍体冷冻胚胎移植的患者的流产率？

目的

确定接受单倍体冷冻胚胎移植 (SEFET) 的患者囊胚线粒体 DNA (mtDNA) 含量是否与流产率相关。

方法

2017年4月至2018年12月期间，对总共355个单整倍体冷冻胚胎移植周期进行了回顾性研究。对第5/6天的囊胚进行了滋养外胚层活检。新一代测序（NGS）后，mtDNA含量计算为线粒体DNA与核DNA的比率，并评估囊胚mtDNA含量与流产率之间的关联。

结果）

在 314 名患者中选择了 355 个整倍体囊胚进行 SEFET，平均年龄为 33.7 ± 5.6 岁；第 5 天对 255 例（71.8%）进行了活检，第 6 天对 100 例进行了活检（28.2%）。冷冻胚胎移植（FET）在激素替代疗法（HRT）周期（71.8%； n  = 255）或自然周期（NC）（28.2%；n = 100）中进行 。妊娠率为 66.2% (235/355)，临床妊娠率和流产率分别为 52.4% ( n  = 186) 和 5.6% ( n  = 20)。妊娠与非妊娠患者囊胚mtDNA含量（27.7±9.2 vs. 29.4±8.6，P  = 0.095）以及临床妊娠与流产患者（30.5±9.3 vs. 27.3±9.2，P ）之间均无显着差异。  = 0.136）。多变量逻辑回归分析显示，除了流产率和BMI（OR 1.149，95% CI 1.03–1.28；P  = 0.012）之外，两者之间存在同样的不显着关系。

结论

线粒体 DNA 含量无法预测植入的人类整倍体囊胚的流产。