Hand, foot and mouth disease is a common viral infectious disease caused by enteroviruses, including coxsackie A16 (CVA16) and enterovirus 71 (EV71). HFMD can cause severe symptoms in children which can be fatal. Human scavenger receptor class B member 2 (SCARB2) is a cellular receptor for EV71 and CVA16, providing a potential approach for preventing EV71 infection and transmission. In this present study, we constructed and assessed the potential of recombinant SCARB2, using E. coli expression system. To generate this construct, scarb2 gene was cloned into pET22b vector and expressed in E. coli BL21 (DE3). The expression of SCARB2 was induced by 0.1 mM IPTG and analyzed using SDS-PAGE, followed by Western blot. Expressed SCARB2 was in inclusion bodies and refolded to obtain the soluble form with recovery efficacy of 100%. This recombinant protein was then validated for binding with EV71 via indirect ELISA in two different pHs (7.4 and 5.5), which partially revealed the mechanism of virus–receptor interaction. These results envisaged potential applications for utilizing recombinant SCARB2 in preventing the virus transmission.

手足口病是由肠道病毒引起的常见病毒性传染病，包括柯萨奇A16（CVA16）和肠道病毒71（EV71）。手足口病可导致儿童出现严重症状，甚至可能致命。人类清道夫受体 B 类成员 2 (SCARB2) 是 EV71 和 CVA16 的细胞受体，为预防 EV71 感染和传播提供了潜在的方法。在本研究中，我们使用大肠杆菌表达系统构建并评估了重组 SCARB2 的潜力。为了生成这个构建体，将 Scarb2基因克隆到 pET22b 载体中并在大肠杆菌中表达BL21 (DE3)。SCARB2 的表达由 0.1 mM IPTG 诱导，并使用 SDS-PAGE 进行分析，然后进行蛋白质印迹。表达的SCARB2在包涵体中并重新折叠以获得具有100％回收效率的可溶形式。然后通过间接 ELISA 在两种不同的 pH 值（7.4 和 5.5）下验证该重组蛋白与 EV71 的结合，这部分揭示了病毒-受体相互作用的机制。这些结果设想了利用重组 SCARB2 预防病毒传播的潜在应用。