Marine crude oil spill is the most alarming environmental issue and the dreadful instance of petroleum hydrocarbon pollution around the world. Successful remediation of these toxic pollutants demands for the ample production of biosurfactant and biocatalysts by the native hydrocarbon-degrading microbes. The main focus of this investigation is to determine the potential of a halo-tolerant and biosurfactant-producing hydrocarbonoclastic bacterium, Enterobacter hormaechei, for the effective bioremediation of marine oil spill. The strain had the ability to produce biosurfactant with excellent surface and emulsification activities along with biocatalysts. The activity of the extracellular enzymes such as lipase and laccase was found to be 160 U/ml and 38 U/ml, respectively, whereas the intracellular enzymes like alkane hydroxylase, alcohol dehydrogenase and esterase showed the corresponding activities of 48 U/ml, 86 U/ml and 102 U/ml. Overall, E. hormaechei could be able to degrade nearly 85% of petroleum hydrocarbons present in crude oil within 10 days of incubation. The biosurfactant was characterized to be an anionic, high molecular weight (48 kDa) lipoprotein-type biosurfactant. The biosurfactant was further characterized by Fourier transform infrared spectroscopy. The E. hormaechei was employed for the treatment of simulated marine oil spill and the degradation followed pseudo-second-order kinetics with rate constant k₂ 0.2775 and R² 0.9923. The crude oil degradation was confirmed by gas chromatography–mass spectrometry. The study suggested that the E. hormaechei is a potential biosurfactant and biocatalysts producer with the effective management of accidental marine oil spills.

海上原油泄漏是最令人震惊的环境问题，也是全世界石油碳氢化合物污染的可怕实例。这些有毒污染物的成功补救要求天然​​降解烃的生物大量生产生物表面活性剂和生物催化剂。这项研究的主要重点是确定耐盐和生产生物表面活性剂的碳氢碎裂细菌霍马氏肠杆菌的潜力。，用于海洋溢油的有效生物修复。该菌株具有与生物催化剂一起生产具有优异表面和乳化活性的生物表面活性剂的能力。脂酶和漆酶等细胞外酶的活性分别为160 U / ml和38 U / ml，而烷烃羟化酶，醇脱氢酶和酯酶等细胞内酶分别具有48 U / ml，86的活性。 U / ml和102 U / ml。总体而言，霍马埃希氏菌可以在孵化后10天内降解原油中近85％的石油烃。该生物表面活性剂的特征在于是阴离子型高分子量（48 kDa）脂蛋白型生物表面活性剂。通过傅立叶变换红外光谱进一步表征了生物表面活性剂。的E. hormaechei被用于处理模拟海洋溢油，其降解遵循伪二级动力学，速率常数k _2为0.2775，R ^2为0.9923。通过气相色谱-质谱法确认了原油的降解。该研究表明，霍马埃希氏菌是一种潜在的生物表面活性剂和生物催化剂生产商，可以有效管理意外的海洋溢油。