Background

MicroRNAs play important roles in the pathogenesis of human diseases by regulating target gene expression in specific cells or tissues. Previously, we identified microRNA 452 (MIR452), which was specifically up-regulated in early stage human colorectal cancer (CRC) tissue.

Objective

The current study aims to identify and verify the target genes of MIR452 associated with CRC.

Methods

A luciferase reporter system was used to confirm the effect of MIR452 on ASB8, NOL8, and CDR2 expression. The expression levels of MIR452 and the target genes were evaluated by quantitative RT-PCR (qRT-PCR) and western blotting.

Results

We verified the association between MIR452 and three genes, ASB8, NOL8, and CDR2, and showed that their transcripts were down-regulated by MIR452. Up-regulated MIR452 also down-regulated ASB8, NOL8, and CDR2 mRNA and protein levels in CRC cells. CDR2 protein expression was decreased in CRC tissues compared to adjacent non-tumor tissues.

Conclusions

These results suggest that ASB8, NOL8, and CDR2 were target genes of MIR452 in CRC cells and that up-regulated MIR452 in CRC tissue regulated ASB8, NOL8, and CDR2 expression during colorectal carcinogenesis.

中文翻译：

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MicroRNA 452调节结直肠癌细胞中ASB8、NOL8和CDR2的表达

背景

MicroRNA 通过调节特定细胞或组织中的靶基因表达，在人类疾病的发病机制中发挥重要作用。以前，我们确定了 microRNA 452 ( MIR452 )，它在早期人类结直肠癌 (CRC) 组织中特异性上调。

客观的

目前的研究旨在识别和验证与 CRC 相关的MIR452的靶基因。

方法

荧光素酶报告系统用于确认MIR452对ASB8、NOL8和CDR2表达的影响。通过定量RT-PCR（qRT-PCR）和蛋白质印迹评估MIR452和靶基因的表达水平。

结果

我们验证了MIR452与三个基因ASB8、NOL8和CDR2之间的关联，并表明它们的转录物被MIR452 下调。上调的MIR452还下调了 CRC 细胞中的ASB8、NOL8和CDR2 mRNA 和蛋白质水平。与相邻的非肿瘤组织相比，CRC 组织中的 CDR2 蛋白表达降低。

结论

这些结果表明，ASB8，NOL8，和CDR2被的靶基因MIR452中CRC细胞和上调的MIR452在CRC组织大肠癌发生过程调节ASB8，NOL8，和CDR2表达。