One of the most significant public health challenges of the twenty-first century is the prevention and treatment of infectious diseases caused by various microorganisms, including resistant bacteria and viruses. With aging, there is a progressive alteration of the immune system and its responses that may result in immune senescence and immunodepression. Nevertheless, immune modulators can speed up recovery. IMMODIN^® is a commercially available dialysable leukocyte extract prepared from disintegrated white blood cells of healthy human donors. It is used clinically as a prophylactic or therapeutic agent when cell-mediated immunity plays a key role. This initial proteomic study was aiming to evaluate the polypeptide repertoire present in this immunologic preparation. Using our approach, we identified forty-eight unique proteins associated with blood cells or plasma. From them, twenty-eight were detected in at least two independent analyses of three IMMODIN^® preparations. The highest number of proteins was related to innate immunity. There were identified receptors for parasites or microbes that have the ability to recognize the invaders and initiate their inhibition or elimination. We also detected proteins associated with inflammatory response, including those having the potential to inhibit cytokines and treat the cytokine storm. There were found even proteins that can speed up recovery by regulating cell growth and repair.

二十世纪最重大的公共卫生挑战之一是预防和治疗由各种微生物（包括抗药性细菌和病毒）引起的传染病。随着年龄的增长，免疫系统及其反应的进行性改变可能导致免疫衰老和免疫抑制。但是，免疫调节剂可以加快恢复速度。IMMODIN ^®是从健康人供体的分解白细胞制备的可商购的可透析白细胞提取物。当细胞介导的免疫起关键作用时，它在临床上用作预防或治疗剂。最初的蛋白质组学研究旨在评估该免疫制剂中存在的多肽库。使用我们的方法，我们鉴定了48种与血细胞或血浆相关的独特蛋白质。从他们身上，28三个IMMODIN至少两个独立的分析检测^®准备。蛋白质的最高数量与先天免疫有关。鉴定出了具有识别入侵者并启动其抑制或消除能力的寄生虫或微生物受体。我们还检测了与炎症反应相关的蛋白质，包括具有抑制细胞因子和治疗细胞因子风暴潜力的蛋白质。甚至发现可以通过调节细胞生长和修复来加速恢复的蛋白质。