当前位置: X-MOL 学术Biomol. NMR Assign. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Resonance assignments of the cytoplasmic domain of ECF sigma factor W pathway protein YsdB from Bacillus subtilis
Biomolecular NMR Assignments ( IF 0.8 ) Pub Date : 2021-01-04 , DOI: 10.1007/s12104-020-09990-3
Yanqing Li 1 , GuangLin Li 1 , Zhihao Wang 1, 2 , Wei Chen 1 , Hongliang Wang 1 , Yawen Wang 1 , Bing Liu 1, 2, 3
Affiliation  

Bacterial sigma (σ) factor, along with RNA polymerase core enzyme, initiates gene transcription from specific promoter regions and therefore regulates clusters of genes in response to a particular situation. The extracytoplasmic function (ECF) σ factors are a class of alternative σ factors that are often associated with environmental signal transduction across the bacterial membrane, in which external signal triggers the release of active σ from the membrane-anchored anti-σ factor. Gram-positive model organism Bacillus subtilis (B. subtilis) has seven ECF σ factors: σM, σV, σX, σW, σY, σZ and σYlaC. Although all these ECF σ factors were found to be involved in B. subtilis antibiotic resistance, σW is among the most studied and considered to play a pivotal role in responding to antimicrobial stresses. σW is under tight control and remains deactivated until exposure to external stimuli, after which proteases PrsW and RasP cleave the specific anti-sigma factor—RsiW to release and activate σW. Membrane anchored protein YsdB is a negative regulator of this activation, possibly via its direct interaction with PrsW and/or RsiW. Importantly, YsdB is well conserved among Bacilli, including pathogenic bacteria like Bacillus cereus. In this study, we describe the chemical shift assignments of the cytoplasmic domain of YsdB (29-130) of B. subtilis in solution as a basis for further interaction studies and structure determination. The near-complete assignment and the solution structure that will follow could provide a further understanding in σW regulation.



中文翻译:


枯草芽孢杆菌 ECF σ 因子 W 途径蛋白 YsdB 胞质结构域的共振分配



细菌西格玛 (σ) 因子与 RNA 聚合酶核心酶一起从特定启动子区域启动基因转录,从而调节基因簇以响应特定情况。胞质外功能(ECF)σ因子是一类替代σ因子,通常与跨细菌膜的环境信号转导相关,其中外部信号触发膜锚定的抗σ因子释放活性σ。革兰氏阳性模式生物枯草芽孢杆菌( B. subtilis ) 具有七个 ECF σ 因子:σ M 、σ V 、σ X 、σ W 、σ Y 、σ Z和 σ YlaC 。尽管所有这些 ECF σ 因子都被发现与枯草芽孢杆菌抗生素耐药性有关,但 σ W是研究最多的因子之一,并被认为在应对抗菌应激方面发挥着关键作用。 σ W受到严格控制并保持失活状态,直到暴露于外部刺激,之后蛋白酶 PrsW 和 RasP 裂解特定的抗 Sigma 因子 RsiW 以释放并激活 σ W 。膜锚定蛋白 YsdB 是这种激活的负调节因子,可能是通过其与 PrsW 和/或 RsiW 的直接相互作用实现的。重要的是,YsdB 在芽孢杆菌中非常保守,包括蜡状芽孢杆菌等病原菌。在本研究中,我们描述了溶液中枯草芽孢杆菌YsdB (29-130) 细胞质结构域的化学位移分配,作为进一步相互作用研究和结构测定的基础。 接近完成的作业和随后的解决方案结构可以提供对 σ W调节的进一步理解。

更新日期:2021-01-05
down
wechat
bug