Amphibians are organisms that suffer high damage from ecological imbalance, since they necessarily have aquatic reproduction and larval phase, being, due to the fragility of this development stage, more susceptible to harmful changes in the environment. The occurrence of environmental hypoxia during development can play an important role in the involvement of serious abnormalities of the central nervous system and heart defects. Bullfrog eggs (Lithobates catesbeianus) (n = 24) were kept in aquariums, with a volume of 5 L, and were divided into two experimental groups: Control Group and Hypoxia Group. The treated groups were kept in experimental conditions during the initial two days of development, being then relocated to aquariums with dechlorinated water and constant aeration. After 12 days the tadpoles had their measurements of mass and length taken and then they were anesthetized by hypothermia, euthanized, and fixed for histological preparation. Animals exposed to hypoxia showed a reduction in body mass and length, as well as a decrease in the mean thickness of the trabeculae of the cardiac tissue. The results show that the acute exposure of bullfrog tadpoles to severe hypoxia has deleterious effects on their development.

两栖动物是受到生态失衡严重破坏的生物，因为它们必须具有水生繁殖和幼体期，由于该发育阶段的脆弱性，它们更容易受到环境有害变化的影响。发育过程中环境缺氧的发生可能在中枢神经系统严重异常和心脏缺陷的发生中起重要作用。牛蛙卵（Lithobates catesbeianus）（n = 24）放在容量为5 L的水族馆中，并分为两个实验组：对照组和低氧组。在开发的最初两天中，将处理过的组保持在实验条件下，然后将其移至装有脱氯水并不断通风的水族馆中。12天后，测量measurements的质量和长度，然后通过体温过低麻醉，安乐死并固定以进行组织学制备。暴露于缺氧状态的动物体重减轻，体长减少，心脏组织小梁的平均厚度减小。结果表明，牛蛙t急性暴露于严重的缺氧状态会对它们的发育产生有害影响。