Growth kinetics of a Vero cells adapted Bangladeshi strain of peste des petits ruminants virus was studied in Vero cells to determine maximum virus yield. One-step growth curve was formulated after determining virus in both supernatant (CFV) and cell lysate (CAV) at different time categories by microtitre plate titration in Vero cells and the viral presence was confirmed by real-time RT-PCR. The virus was first detected in both the supernatants and cell pellets at 12 hpi. The virus titre reached its plateau at 72 hpi. Maximum virus titre of CAV was 6.2 log10 TCID50/ml and that of CFV was 5.2 log10 TCID50/ml at 72 hpi. After that, the titer gradually declined, but maintained at 4.5 log10 TCID50/ml in case of CAV and 4.2 log10 TCID50/ml in case of CFV at 96 hpi. It was concluded that the optimum time point for harvesting Vero cell culture is 72 hpi.

中文翻译：

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Vero 细胞在细胞培养中适应孟加拉小反刍兽疫 (PPR) 病毒株的生长动力学

在 Vero 细胞中研究了适应了孟加拉鼠疫病病毒株的 Vero 细胞的生长动力学，以确定最大的病毒产量。在 Vero 细胞中通过微量滴定板滴定确定不同时间类别的上清液 (CFV) 和细胞裂解物 (CAV) 中的病毒后，制定了一步生长曲线，并通过实时 RT-PCR 确认病毒的存在。病毒首先在感染后 12 小时在上清液和细胞沉淀中检测到。病毒滴度在 72 hpi 时达到了平台期。在 72 hpi 时，CAV 的最大病毒滴度为 6.2 log10 TCID50/ml，CFV 的最大病毒滴度为 5.2 log10 TCID50/ml。之后，滴度逐渐下降，但在 96 hpi 时在 CAV 的情况下保持在 4.5 log10 TCID50/ml，在 CFV 的情况下保持在 4.2 log10 TCID50/ml。得出的结论是，收获 Vero 细胞培养物的最佳时间点是 72 hpi。