In this study, two quorum sensing (QS) system genes, las and rhI; N-3-oxo-dodecanoyl homoserine lactone (AHL; 3-O-C12-HSL); and QS-related virulence factors and correlation between them were assessed in 30 fish origin P. aeruginosa isolates. The detection of two QS system of the isolates, and eight gene regions consisting of four intact (lasI/R, rhlI/R) and four internal (lasI/R, rhlI/R) genes were tested by PCR assay. According to findings, las and rhI QS system genes were detected in 27 and 30 isolates, respectively, while 3-O-C12-HSL was determined in 13 isolates. A total of 22, 27, and 18 isolates were capable of pyocyanin production, protease, and elastase activity, respectively. Biofilm formation was detected using three methods in all 30 isolates: 12 by Congo red agar, 14 by microtiter plate, and 29 by tube test. Twitching and swarming motility types were detected in 30, but the swimming motility was determined in 25 isolates. The rhI QS system genes detected in all of the isolates having three types including motility, PYA production, and protease and elastase activities. The las QS system genes were detected in 27 of the motility, 17 of PYA production, 25 of protease, and 16 of elastase activity having isolates. In conclusion, the high number of P. aeruginosa isolates from fish tested have two QS systems and related virulence factors. There was also correlation between them.

中文翻译：

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从鱼类中分离出的铜绿假单胞菌中的群体感应系统、相关毒力因子和生物膜形成

在这项研究中，两个群体感应 (QS) 系统基因 las 和 rhI；N-3-氧代-十二烷酰基高丝氨酸内酯（AHL；3-O-C12-HSL）；和 QS 相关的毒力因子以及它们之间的相关性在 30 个鱼类来源的铜绿假单胞菌分离株中进行了评估。PCR法检测了分离株的2个QS系统和由4个完整基因(lasI/R、rhlI/R)和4个内部(lasI/R、rhlI/R)基因组成的8个基因区域。根据研究结果，分别在 27 和 30 个分离株中检测到 las 和 rhI QS 系统基因，而在 13 个分离株中检测到 3-O-C12-HSL。总共有 22、27 和 18 个分离株能够分别产生绿脓素、蛋白酶和弹性蛋白酶活性。使用三种方法在所有 30 个分离株中检测到生物膜形成：12 个通过刚果红琼脂，14 个通过微量滴定板，29 个通过试管测试。在 30 个分离物中检测到抽搐和蜂群运动类型，但在 25 个分离物中确定了游泳运动。在所有分离株中检测到的 rhI QS 系统基因具有三种类型，包括运动性、PYA 产生以及蛋白酶和弹性蛋白酶活性。las QS 系统基因在 27 个运动性、17 个 PYA 生产、25 个蛋白酶和 16 个具有分离物的弹性蛋白酶活性中检测到。总之，从测试的鱼类中分离出的大量铜绿假单胞菌具有两个 QS 系统和相关的毒力因子。它们之间也存在相关性。las QS 系统基因在 27 个运动性、17 个 PYA 生产、25 个蛋白酶和 16 个具有分离物的弹性蛋白酶活性中检测到。总之，从测试的鱼类中分离出的大量铜绿假单胞菌具有两个 QS 系统和相关的毒力因子。它们之间也存在相关性。las QS 系统基因在 27 个运动性、17 个 PYA 生产、25 个蛋白酶和 16 个具有分离物的弹性蛋白酶活性中检测到。总之，从测试的鱼类中分离出的大量铜绿假单胞菌具有两个 QS 系统和相关的毒力因子。它们之间也存在相关性。