Species belonging to the genus Artemisia have high economic value and are used in folk and modern medicine. Analysis and identification of their genetic relationships can help in efficient conservation and further utilization of Artemisia genetic resources. This study was conducted to evaluate the genetic diversity among seven species of the genus Artemisia using their morphological characteristics and inter-simple sequence repeat (ISSR)-based molecular markers. The morphology of leaf and achene was examined by light microscopy and scanning electron microscopy. Morphological and molecular data were analyzed using the multivariate statistical package program. There were obvious variations in the morphology of leaf and achene among the Artemisia species. The ISSR results showed a total of 173 amplified loci, consisting of 132 polymorphic loci and 41 unique loci. Polymorphism percentage ranged from 62.5 to 88.8% for nine primers. Mean polymorphism information content for ISSR markers was 0.88, which suggested the efficacy of the ISSR marker system in determining polymorphisms. Significant correlation (r = 0.56, P = 0.004) was observed between similarity coefficients of morphological parameters and ISSR data based on the Mantel test. A wide range of diversity was observed at both morphological and molecular levels among the species. Genetic analyses using ISSR allowed for the detection of relationships between various Artemisia species than was apparent by the analyses of morphological characteristics of these species. Moreover, a mixed pattern of grouping in cluster analysis was found, indicating the close affinities of species with each other and also that the section Artemisia is polyphyletic.

属于蒿属的种具有很高的经济价值，被用于民间医学和现代医学。分析和鉴定它们的遗传关系可以帮助有效保存和进一步利用蒿属遗传资源。进行了这项研究，以评估蒿属（Artemisia）的七个物种的形态特征和基于简单序列重复（ISSR）的分子标记。通过光学显微镜和扫描电子显微镜检查叶片和瘦果的形态。使用多元统计软件包程序分析形态和分子数据。青蒿叶片和瘦果形态差异明显。种类。ISSR结果显示总共有173个扩增基因座，由132个多态性基因座和41个独特基因座组成。九种引物的多态性百分比范围为62.5至88.8％。ISSR标记的平均多态性信息含量为0.88，表明ISSR标记系统在确定多态性方面的功效。 根据Mantel检验，形态参数的相似系数与ISSR数据之间存在显着相关性（r  = 0.56，P = 0.004）。在物种的形态和分子水平上都观察到了广泛的多样性。使用ISSR进行遗传分析可检测出各种蒿之间的关系通过分析这些物种的形态特征可以明显看出这些物种。此外，在聚类分析中发现了一种混合的分组模式，这表明物种之间的亲和力很强，而且蒿属部分是多系的。