Data were collected from 30 strains of Vibrio and sampled on different organs (brain, hemorrhagic site and digestive tract) of Sciaenops ocellatus infection. The results showed that the nucleotide sequences 16S rRNA region are highly similar to those of V. alginolyticus, V. azureus, V. fluvialis, V. natriengens and V. orientalis, which were published on Genbank and other, ranging from 98.05 to 100%. The digestive tract has the most common Vibrio strains (V. alginolyticus [16] V. azureus [7] and V. fluvialis). Thereout, 25 of 30 strains of Vibrio contained 1 to 3 toxin genes, except V. parahaemolyticus. Six parameters were used to measure the DNA polymorphism of 33 homologous DNA sequences in this Vibrio bacteria population. The results indicated that number of separate polymorphic sites (S), total number of mutant sites (Eta), number of haplotype (h), haplotype diversity (Hd), average number of nucleotide differences (k), nucleotide diversity (Pi) were 98 (S), 103 (Eta), 9 (h), 0.887 ± 0.032 (Hd), 25.789 (k) and 17.980 × 10–3 ± 0.003 (Pi), respectively (P < 0,05). The G + C content above 1434 sites positions of nucleotide sequences accounted for 0.542. The phylogenetic tree showed that these strains are divided into six groups. As observed, the appearance of isolated Vibrio on 3 organs of fish (S. ocellatus) hemorrhagic are V. azureus (27,67%), V. alginolyticus (50%), V. orientalis (6,67%) and V. fluvialis (16,67%). Through this result, we found that the diversity of Vibrio species that appeared on the red drum was used in the 16S rRNA region and the presence of toxin genes in these Vibrio species.

从 30 株弧菌中收集数据，并在Sciaenops ocellatus感染的不同器官（大脑、出血部位和消化道）上取样。结果表明，16S rRNA区域的核苷酸序列与Genbank等发表的溶藻弧菌、天蓝色弧菌、河豚弧菌、纳特里根弧菌和东方弧菌高度相似，98.05～100% . 消化道有最常见的弧菌菌株（溶藻弧菌[16] V. azureu s [7] 和V. fluvialis）。其中，30 株弧菌中有 25 株除副溶血性弧菌外，含有 1 至 3 个毒素基因。六个参数用于测量该弧菌群中 33 个同源 DNA 序列的 DNA 多态性。结果表明，分离多态位点数（S）、突变位点总数（Eta）、单倍型数（h）、单倍型多样性（Hd）、平均核苷酸差异数（k）、核苷酸多样性（Pi）分别为98 (S), 103 (Eta), 9 (h), 0.887 ± 0.032 (Hd), 25.789 (k) 和 17.980 × 10–3 ± 0.003 (Pi) (P < 0,05)。核苷酸序列1434个位点以上的G+C含量占0.542。系统发育树显示这些菌株分为六组。正如观察到的，在鱼的 3 个器官上出现了孤立的弧菌（S. ocellatus ) 出血性V. azureus (27,67%), V. alginolyticus (50%), V. orientalis (6,67%) 和V. fluvialis (16,67%)。通过这个结果，我们发现出现在红鼓上的弧菌物种的多样性被用于16S rRNA区域以及这些弧菌物种中毒素基因的存在。