The newly isolated Trametes polyzona PBURU 12 demonstrated a high tolerance and potential for the degradation of phenanthrene. The fungal isolate was able to tolerate 100 ppm of phenanthrene with 45% relative growth. The crude laccase produced by Trametes polyzona PBURU 12 was able to degrade phenanthrene by up to 98% within 24 h. The degradation metabolites showed the absence of toxic compounds. Microbial viability tests using E. coli and B. subtilis revealed that the treated phenanthrene was less toxic than untreated phenanthrene. Phytotoxicity and genotoxicity tests, using Vigna radiata and Allium cepa, indicated that the treated phenanthrene was less toxic to the plants. No mutagenic activity was found in the Ames test. The crude laccase from Trametes polyzona PBURU 12 was demonstrated as a potential tool for the biodegradation of PAHs (phenanthrene), with low toxic effects after the degradation.

新分离的Trametes polyzona PBURU 12 表现出对菲降解的高耐受性和潜力。真菌分离物能够耐受 100 ppm 的菲，相对增长 45%。Trametes polyzona PBURU 12生产的粗漆酶能够在 24 小时内降解高达 98% 的菲。降解代谢物显示不存在有毒化合物。使用大肠杆菌和枯草芽孢杆菌进行的微生物活力测试表明，经处理的菲比未经处理的菲毒性更小。植物毒性和遗传毒性试验，使用豇豆和洋葱，表明处理过的菲对植物的毒性较小。在 Ames 试验中未发现致突变活性。来自Trametes polyzona PBURU 12的粗漆酶被证明是多环芳烃（菲）生物降解的潜在工具，降解后具有低毒性。