ZnO/SiO2 core/shell nanowires for capturing CpG rich single-stranded DNAs,Analytical Methods

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ZnO/SiO2 core/shell nanowires for capturing CpG rich single-stranded DNAs
Analytical Methods ( IF 2.7 ) Pub Date : 2020-12-21 , DOI: 10.1039/d0ay02138e
Marina Musa _{1,

2,

3,

4,

5} , Takao Yasui _{1,

2,

3,

4,

5} , Kazuki Nagashima _{5,

6,

7,

8,

9} , Masafumi Horiuchi _{1,

2,

3,

4,

5} , Zetao Zhu _{1,

2,

3,

4,

5} , Quanli Liu _{1,

2,

3,

4,

5} , Taisuke Shimada _{1,

2,

3,

4,

5} , Akihide Arima _{1,

2,

3,

4,

5} , Takeshi Yanagida _{2,

5,

9,

10,

11} , Yoshinobu Baba _{1,

2,

3,

4,

5}

Affiliation

Atomic layer deposition (ALD) is capable of providing an ultrathin layer on high-aspect ratio structures with good conformality and tunable film properties. In this research, we modified the surface of ZnO nanowires through ALD for the fabrication of a ZnO/SiO₂ (core/shell) nanowire microfluidic device which we utilized for the capture of CpG-rich single-stranded DNAs (ssDNA). Structural changes of the nanowires while varying the number of ALD cycles were evaluated by statistical analysis and their relationship with the capture efficiency was investigated. We hypothesized that finding the optimum number of ALD cycles would be crucial to ensure adequate coating for successful tuning to the desired surface properties, besides promoting a sufficient trapping region with optimal spacing size for capturing the ssDNAs as the biomolecules traverse through the dispersed nanowires. Using the optimal condition, we achieved high capture efficiency of ssDNAs (86.7%) which showed good potential to be further extended for the analysis of CpG sites in cancer-related genes. This finding is beneficial to the future design of core/shell nanowires for capturing ssDNAs in biomedical applications.

中文翻译：

ZnO / SiO2核/壳纳米线用于捕获富含CpG的单链DNA

原子层沉积（ALD）能够在高纵横比的结构上提供具有良好的保形性和可调膜特性的超薄层。在这项研究中，我们通过ALD修饰了ZnO纳米线的表面，以制造ZnO / SiO ₂（核/壳）纳米线微流体装置，我们用于捕获富含CpG的单链DNA（ssDNA）。通过统计分析评估了纳米线在改变ALD循环次数时的结构变化，并研究了它们与捕获效率的关系。我们假设找到最佳数量的ALD循环对于确保足够的涂层以成功调整至所需的表面性能至关重要，除了促进生物分子穿过分散的纳米线时，以最佳的间隔大小捕获足够的捕获区域以捕获ssDNA之外。在最佳条件下，我们实现了ssDNA的高捕获效率（86.7％），这显示出有很好的潜力可进一步用于分析癌症相关基因中的CpG位点。

更新日期：2021-01-04

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