ABSTRACT

1. The long noncoding RNA lncGLM is significantly differentially expressed in the livers of peak-laying hens compared with that in the livers of pre-laying hens, but its potential biological role and expression regulation are unclear.

2. To explore the potential biological function of lncGLM, single nucleotide polymorphism (SNP) detection and association analysis were carried out in the Gushi×Anka F₂ resource population.

3. The tissues and spatiotemporal expression characteristics of lncGLM were analysed by real-time quantitative PCR. The effects of 17β-oestradiol on the expression of lncGLM expression were analysed through in vitro and in vivo experiments.

4. The results showed that a g.19069338 T > C SNP was present in lncGLM. Association analysis revealed that lncGLM was significantly associated with body slanting length at 12 weeks, body weight at 12 weeks, shank length at four weeks, chest depth at eight weeks, pelvic width at 12 weeks, eviscerated weight, head weight, pancreas weight, pectoralis weight, leg muscle weight, muscular stomach weight rate, pancreas weight rate, carcase weight, aspartate aminotransferase, creatinine and pectoral muscle water loss rate.

5. The expression of lncGLM in the liver was higher than that in other sampled tissues. In addition, the expression of lncGLM in the liver was significantly higher in the peak-laying period than at the pre-laying period. Both in vitro and in vivo experiments showed that lncGLM expression was regulated by 17β-oestradiol via oestrogen receptor alpha (ER-α). These results demonstrated that the chicken lncGLM gene is highly expressed in liver tissue and regulated by oestrogen through ER-α.

摘要

1.长峰非编码RNA lncGLM在产蛋高峰期的母鸡肝脏中的表达与产蛋前期母鸡的肝脏明显不同，但尚不清楚其潜在的生物学作用和表达调控。

2.为了探讨lncGLM的潜在生物学功能，对固始×安卡F ₂资源种群进行了单核苷酸多态性（SNP）检测和关联分析。

3.通过实时定量PCR分析lncGLM的组织和时空表达特征。通过体外和体内实验分析了17β-雌二醇对lncGLM表达的影响。

4.结果显示在lncGLM中存在g.19069338T > C SNP 。关联分析显示，lncGLM与12周体重，12周体重，4周胫骨长，8周胸深度，12周骨盆宽度，去内脏体重，头部重量，胰腺重量，胸大肌明显相关体重，腿部肌肉重量，肌肉胃部重量率，胰腺重量率，屠体重量，天冬氨酸转氨酶，肌酐和胸肌失水率。

5.肝组织中lncGLM的表达高于其他采样组织。另外，在产蛋高峰期，肝脏中lncGLM的表达明显高于产蛋前。二者在体外和体内试验表明，lncGLM表达通过17β雌二醇调节通过雌激素受体α（ER-α）。这些结果表明，鸡lncGLM基因在肝组织中高表达，并受雌激素通过ER-α调控。