Abstract

Two new sucrose derivatives, named as cannadica A (1) and cannadica B (2), and eight known compounds, 6′-O-acetyl-3-O-(E)-p-coumaroylsucrose (3), heterophylloside C (4), 6′-O-vanilloylarbutin (5), isotachioside (6), 2,6-dimethoxy-p-hydroquinone 1-O-β-D-glucopyranoside (7), 3,5-dimethoxy-p-hydroquinone 1-O-β-D-glucopyranoside (8), benzyl glucoside (9), and 3,4-dihydroxybenzaldehyde (10) were isolated from the roots of Canna indica L. by various chromatographic methods. Their structures were established by extensive spectroscopic analysis (UV, IR, HR-ESI-MS and NMR) and by comparison of the spectral data with those reported in the literature. Compounds 1-10 were evaluated their antioxidant activity by peroxyl radical absorbance capacity assay. Compounds 4 and 5 exhibited the most peroxyl radical absorbance capacity. At concentration of 1 µM, their ORAC_ROO* values were 4.86 ± 0.39 and 3.11 ± 0.26, respectively, fold-up to that of trolox as an internal standard.

摘要

两种新的蔗糖衍生物，命名为 cannadica A ( 1 ) 和 cannadica B ( 2 )，以及八种已知化合物， 6'- O -acetyl -3- O -( E )- p -coumaroyl sucrose ( 3 )、heterophylloside C ( 4 ), 6'- O -vanilloylarbutin ( 5 ), isotachioside ( 6 ), 2,6-dimethoxy- p -hydroquinone 1-O-β-D-glucopyranoside ( 7 ), 3,5-dimethoxy- p - hydroquinone 1- O-β-D-吡喃葡萄糖苷 ( 8 )、苄基葡萄糖苷 ( 9 ) 和 3,4-二羟基苯甲醛 ( 10 )) 通过各种色谱方法从Canna indica L.的根中分离得到。它们的结构是通过广泛的光谱分析（UV、IR、HR-ESI-MS 和 NMR）以及通过将光谱数据与文献中报道的数据进行比较来确定的。化合物1-10通过过氧自由基吸收能力测定来评价它们的抗氧化活性。化合物4和5表现出最大的过氧自由基吸收能力。在 1 µM 的浓度下，它们的 ORAC _ROO*值分别为 4.86 ± 0.39 和 3.11 ± 0.26，与作为内标的 trolox 的倍数相同。