The dynamics of two different fluorescent dyes labelling DNA and cationic liposomes is monitored through Fluorescence Cross‐Correlation Spectroscopy (FCCS). Measuring the extent of correlation between the flickering of the two dyes as they diffuse in and out of overlapping confocal volumes, the association of DNA and liposomes to form lipoplexes is quantified. This was achieved analysing the correlation with non 1:1 stoichiometric sample description.

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封面

通过荧光互相关光谱法（FCCS）监控标记DNA和阳离子脂质体的两种不同荧光染料的动力学。当两种染料在重叠共焦体积中扩散进出扩散时，测量两种闪烁之间的相关程度，对DNA和脂质体形成脂质复合物的缔合进行定量。这是通过分析与非1：1化学计量样品描述的相关性而实现的。