Chemical binding of horseradish peroxidase enzyme with poly beta‐cyclodextrin and its application as molecularly imprinted polymer for the monitoring of H2O2 in human plasma samples,Journal of Molecular Recognition

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Chemical binding of horseradish peroxidase enzyme with poly beta‐cyclodextrin and its application as molecularly imprinted polymer for the monitoring of H2O2 in human plasma samples
Journal of Molecular Recognition ( IF 2.3 ) Pub Date : 2021-01-03 , DOI: 10.1002/jmr.2884
Sanam Sardaremelli _{1,

2} , Mohammad Hasanzadeh ₃ , Habib Razmi ₁

Affiliation

In this study, a selective and sensitive molecular imprinting‐based electrochemical sensors, for horseradish peroxidase (HRP) entrapment was fabricated using electro polymerization of ß‐Cyclodextrin (ß‐CD) on the surface of glassy carbon electrode. Poly beta‐cyclodextrin P(ß‐CD) provide efficient surface area for self‐immobilization of HRP as well as improve imprinting efficiency. The proposed imprinted biosensor successfully utilized for detection of HRP with excellent analytical results which linear range is 0.1 mg/mL to 10 ng/mL with LOD of 2.23 ng/mL. Furthermore, electrocatalytical activity of the prepared biosensor toward the reduction of hydrogen peroxide was investigated in the ranges of 1 to 15 μM with a detection limit of 0.4 μM by using chronoamperometry technique. The developed biosensor was used for the detection of hydrogen peroxide in unprocessed human plasma sample.

中文翻译：

辣根过氧化物酶与聚β-环糊精的化学结合及其作为分子印迹聚合物用于监测人血浆样品中过氧化氢的应用

在这项研究中，使用 ß-环糊精 (ß-CD) 在玻碳电极表面上的电聚合制备了一种选择性和灵敏的基于分子印迹的电化学传感器，用于捕获辣根过氧化物酶 (HRP)。聚 β-环糊精 P(ß-CD) 为 HRP 的自固定提供了有效的表面积并提高了印迹效率。所提出的印迹生物传感器成功地用于检测 HRP，具有出色的分析结果，线性范围为 0.1 mg/mL 至 10 ng/mL，LOD 为 2.23 ng/mL。此外，使用计时电流法研究了制备的生物传感器对过氧化氢还原的电催化活性，范围为 1 至 15 μM，检测限为 0.4 μM。

更新日期：2021-01-03

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