Human adipose‐derived stromal/stem cells (ASC) have immunomodulatory properties and the potential to differentiate into several cell lines, important for application in regenerative medicine. However, the contamination with dermal fibroblasts (FIB) can impair the beneficial effects of ASC in cell therapy. It is then essential to develop new strategies that contribute to the distinction between these two cell types. In this study, we performed functional assays, high‐throughput RNA sequencing (RNA‐Seq) and quantitative PCR (qPCR) to find new markers that can distinguish ASC and FIB. We showed that ASC have adipogenic and osteogenic differentiation capacity and alkaline phosphatase activity, not observed in FIB. Gene expression variation analysis identified more than 2000 differentially expressed genes (DEG) between these two cell types. We validated 16 genes present in the list of DEG, including the alkaline phosphatase gene (ALPL). In conclusion, we showed that ASC and FIB have distinct biological properties as demonstrated by alkaline phosphatase activity and differentiation capacity, besides having different gene expression profiles.

中文翻译：

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通过生物学表征和RNA测序评估，人脂肪来源的基质/干细胞不同于真皮成纤维细胞

人脂肪来源的基质/干细胞（ASC）具有免疫调节特性，并具有分化成几种细胞系的潜力，这对于再生医学的应用很重要。但是，真皮成纤维细胞（FIB）的污染会损害ASC在细胞治疗中的有益作用。因此，至关重要的是开发有助于区分这两种细胞类型的新策略。在这项研究中，我们进行了功能测定，高通量RNA测序（RNA‐Seq）和定量PCR（qPCR），以发现可以区分ASC和FIB的新标记。我们显示，ASC具有成脂和成骨分化能力以及碱性磷酸酶活性，在FIB中未观察到。基因表达变异分析确定了这两种细胞类型之间的2000多个差异表达基因（DEG）。ALPL）。总之，我们显示ASC和FIB除了具有不同的基因表达谱外，还具有独特的生物学特性，如碱性磷酸酶活性和分化能力所证明。