Laser speckle contrast imaging (LSCI) has been widely used for mapping blood flow. LSCI is well known to be highly sensitive to movement artifacts. Previous studies attempted to register the global misalignment by using raw speckle images and rigid registration algorithm. However, it is difficult to correct the local non-rigid motion only by using granular raw speckle images, especially in the tissue dominated by small blood vessels. Here, we propose a method of imaging the tissue structure and its laser speckle simultaneously by a colour camera combined with a non-rigid registration algorithm to improve the motion correction of speckle imaging. The results of animal experiments show that this method significantly reduces the influence of non-rigid, spatially non-uniform motion on the estimation of blood flow, and improves the visualization of blood vessels in moving tissue, which can help to promote the practical application of LSCI technology.

激光斑点对比成像（LSCI）已被广泛用于绘制血流图。众所周知，LSCI对运动伪影高度敏感。先前的研究试图通过使用原始斑点图像和刚性配准算法来配准全局未对准。但是，仅通过使用颗粒状的原始斑点图像来校正局部非刚性运动是困难的，特别是在以小血管为主的组织中。在这里，我们提出了一种通过彩色相机同时结合非刚性配准算法对组织结构及其激光斑点进行成像的方法，以改善斑点成像的运动校正。动物实验结果表明，这种方法可大大减少非刚性，空间不均匀运动对血流估计的影响，