Abiotic stresses widely constrain the development and reproduction of plant, especially impaired the yield of crops greatly. Recent researches presented pentatricopeptide repeat (PPR) proteins play crucial role in response to abiotic stress. However, the underlying mechanism of PPR genes in regulation of abiotic stress is still obscures. In our recent study, we found that the knockout of rice PPS1 causes pleiotropic growth disorders, including growth retardation, dwarf and sterile pollen, and finally leads to impaired C-U RNA editing at five consecutive sites on the mitochondrial nad3. In this study, we further investigate the roles of PPS1 in abiotic stress tolerance, we confirmed that pss1-RNAi line exhibited enhanced sensitivity to salinity and ABA stress at vegetative stage, specifically. While reactive oxygen species (ROS) accumulate significantly only at reproductive stage, which further activated the expression of several ROS-scavenging system related genes. These results implied that PPS1 functioned on ROS signaling network to contribute for the flexibility to abiotic stresses. Our research emphasizes the stress adaptability mediated by the PPR protein, and also provides new insight into the understanding of the interaction between cytoplasm and nucleus and signal transduction involved in RNA editing.

中文翻译：

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水稻 PPR 基因 PPS1 的沉默表现出对非生物胁迫的敏感性增强和显着的 ROS 积累

非生物胁迫广泛地限制了植物的发育和繁殖，特别是对作物产量的影响很大。最近的研究表明，五肽重复序列 (PPR) 蛋白在应对非生物胁迫中起着至关重要的作用。然而，PPR基因调控非生物胁迫的潜在机制仍不清楚。在我们最近的研究中，我们发现敲除水稻 PPS1 会导致多效性生长障碍，包括生长迟缓、矮化和不育花粉，并最终导致线粒体 nad3 上五个连续位点的 CU RNA 编辑受损。在这项研究中，我们进一步研究了 PPS1 在非生物胁迫耐受中的作用，我们证实 pss1-RNAi 系在营养阶段表现出对盐度和 ABA 胁迫的增强敏感性。而活性氧 (ROS) 仅在生殖阶段显着积累，这进一步激活了几个 ROS 清除系统相关基因的表达。这些结果表明 PPS1 在 ROS 信号网络上起作用，有助于对非生物胁迫的灵活性。我们的研究强调了 PPR 蛋白介导的应激适应性，也为理解细胞质和细胞核之间的相互作用以及 RNA 编辑中涉及的信号转导提供了新的见解。