The exploitation of natural origin macromolecules, as complex physical mixtures or drugs, increases in biomedical or tissue engineering (TE) solutions. Aloe Vera is a highly explored medicinal plant, from which the main polysaccharide is acemannan (ACE). The ACE combination with chitosan and alginate results in interactions that lead to mixed junction zones formation, predicting membrane functionality improvement.

This work proposes the development and characterization of ACE-based blended films as a promising strategy to design a nature-derived bioactive platform.

The results confirmed that stable complex polyelectrolyte structures were formed through different intermolecular interactions. The films present good dimensional stability, flexibility, an adequate swelling ability with mostly radial water uptake, and a sustainable ACE release to the medium. Positive biological performance of the ACE-based blended films with L929 cells also suggested that they can be applied in TE solutions, with the potential to act as bioactive topical platforms.

天然来源的大分子作为复杂的物理混合物或药物的开发，在生物医学或组织工程学（TE）解决方案中的应用越来越多。芦荟是一种高度探索的药用植物，其主要多糖是乙酰甘露聚糖（ACE）。ACE与壳聚糖和藻酸盐的结合导致相互作用，导致混合连接区的形成，预示着膜功能的改善。

这项工作提出了基于ACE的混合膜的开发和表征，作为设计自然衍生的生物活性平台的一种有前途的策略。

结果证实了稳定的复合高分子电解质结构是通过不同的分子间相互作用形成的。这些薄膜具有良好的尺寸稳定性，柔韧性，足够的溶胀能力（主要是径向吸收水）和可持续的ACE释放到介质中。带有L929细胞的基于ACE的混合膜的积极生物学性能也表明，它们可用于TE解决方案，具有充当生物活性局部平台的潜力。