Abstract

The electrochemical oxidation of pentamidine drug was first investigated in aqueous buffered solutions by cyclic voltammetry using a glassy carbon electrode. Pentamidine exhibited pH dependent irreversible oxidation peaks at high anodic potentials through a diffusion-controlled process as the benzamidine moieties of pentamidine are oxidized to benzamidoxime. Thereafter, the pentamidine-dsDNA interaction was investigated in solution and at dsDNA-sensor surface. The change in the oxidation signals intensity of guanine and adenine before and after interaction with pentamidine was monitored by the differential pulse voltammetry. The decrease in the peak current of their signals was used as an indication for the interaction event. After that, the diazonium chemistry was applied for the immobilization of ds-DNA on glassy carbon electrode surface. The interaction between pentamidine and immobilized DNA were determined by the changes in the voltammetric peak of crystal violet redox probe. The peak currents of the crystal violet decreased after pentamidine interaction with immobilized DNA. Finally, the pentamidine-dsDNA interaction was further assessed by the Faradaic electrochemical impedance spectroscopy, following the changes in charge transfer resistance after immersion of the DNA biosensor in pentamidine solution.

Graphic abstract

中文翻译：

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抗感染剂喷他idine与DNA相互作用的电化学感应

摘要

首先通过使用玻璃碳电极的循环伏安法在缓冲水溶液中研究了喷他drug药物的电化学氧化。当喷他idine的苯甲idine部分被氧化成苯甲酰胺肟时，喷他through通过扩散控制的过程在高阳极电位下显示出pH依赖性的不可逆氧化峰。此后，在溶液中和在dsDNA-传感器表面研究了喷他idine-dsDNA的相互作用。通过差动脉冲伏安法监测与喷他idine相互作用之前和之后鸟嘌呤和腺嘌呤的氧化信号强度的变化。他们的信号的峰值电流的减少被用作相互作用事件的指示。之后，将重氮化学用于将ds-DNA固定在玻璃碳电极表面上。通过结晶紫氧化还原探针伏安峰的变化确定喷他idine与固定的DNA之间的相互作用。戊tam与固定的DNA相互作用后，结晶紫的峰值电流降低。最后，在将DNA生物传感器浸入喷他solution溶液后，电荷转移电阻发生变化，通过法拉第电化学阻抗谱进一步评估了喷他idine与dsDNA的相互作用。

图形摘要