A novel Gram-stain-positive, aerobic, non-spore-forming, and rod-shaped bacterium designated as PW21was isolated from produced water sampled from an oil reservoir in Limbodara, Gujarat, India. Growth occurred at 30–45 °C (optimum 37 °C), at pH 6.5–9.5 (optimum pH 8.5) and in the presence of 0.5–5.0% (w/v) NaCl [optimum, 3% (w/v)]. Phylogenetic analysis based on the 16S rRNA gene sequences showed that PW21^T belonged to the class Actinobacteria, order Actinomycetales, family Promicromonosporaceae, and genus Xylanimonas, with the highest sequence similarity to Isoptericola cucumis AP38 (97.95% with 98.8% completeness), followed by Xylanimonas pachnodae NBRC 107,786^T (97.82%) and Xylanimonas allomyrinae 2JSPR-7^T(97.75%) with 100% completeness for the mentioned strains of Xylanimonas. The genome size of PW21^T was 3.4 Mbp with a G+C content of 73.0 mol% (draft genome sequence). The average nucleotide identity (ANI) value of the draft genomes between PW21^T and related species were found in between 76.99 and 78.93%. Major cellular fatty acids (> 10% of total fatty acids) of PW21^Twere iso-C_15:0, anteiso-C_15:0, and C_16:0. Phylogenetic, physiological, and biochemical characterisation identified strain PW21^T (= KCTC 49,338^T = JCM 33,795^T = MCC 3936^T) as a novel species of the genus Xylanimonas, and hence, name Xylanimonas oleitrophica sp. nov. PW21^T is proposed.

从印度古吉拉特邦 Limbodara 油藏采出的水样中分离出一种新型革兰氏染色阳性、需氧、不形成孢子和杆状细菌，命名为 PW21。生长发生在 30–45 °C（最佳 37 °C）、pH 6.5–9.5（最佳 pH 8.5）和存在 0.5–5.0% (w/v) NaCl [最佳，3% (w/v) ]。基于16S rRNA基因序列的系统发育分析表明，PW21 ^Ť属于类放线菌，为了放线菌目，家庭Promicromonosporaceae，和属Xylanimonas，具有最高的序列相似性Isoptericola黄瓜AP38（97.95％与98.8％完整性），其次是Xylanimonas厚节虫科NBRC 107,786 ^T (97.82%) 和Xylanimonas allomyrinae 2JSPR-7 ^T (97.75%) 对上述木单胞菌菌株具有 100% 的完整性。PW21 ^T的基因组大小为 3.4 Mbp，G+C 含量为 73.0 mol%（基因组序列草案）。PW21 ^T和相关物种之间的基因组草图的平均核苷酸同一性 (ANI) 值介于 76.99 和 78.93% 之间。PW21 ^{T 的}主要细胞脂肪酸（> 10% 的总脂肪酸）是 iso-C _15:0、anteiso-C _15:0和 C _16:0。系统发育、生理和生化特征鉴定菌株 PW21 ^T（= KCTC 49338 ^Ť  = JCM 33795 ^Ť  = MCC 3936 ^Ť）作为属的一个新种Xylanimonas，因此，名称Xylanimonas oleitrophica藻。十一月 建议使用PW21 ^T。