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Xylanimonas oleitrophica sp. nov., a novel petroleum hydrocarbon degrading bacterium isolated from an Indian oil reservoir
Antonie van Leeuwenhoek ( IF 1.8 ) Pub Date : 2021-01-04 , DOI: 10.1007/s10482-020-01506-2
Pradnya D Nagkirti 1, 2 , Anupama S Engineer 1 , Prashant K Dhakephalkar 1, 2
Affiliation  

A novel Gram-stain-positive, aerobic, non-spore-forming, and rod-shaped bacterium designated as PW21was isolated from produced water sampled from an oil reservoir in Limbodara, Gujarat, India. Growth occurred at 30–45 °C (optimum 37 °C), at pH 6.5–9.5 (optimum pH 8.5) and in the presence of 0.5–5.0% (w/v) NaCl [optimum, 3% (w/v)]. Phylogenetic analysis based on the 16S rRNA gene sequences showed that PW21T belonged to the class Actinobacteria, order Actinomycetales, family Promicromonosporaceae, and genus Xylanimonas, with the highest sequence similarity to Isoptericola cucumis AP38 (97.95% with 98.8% completeness), followed by Xylanimonas pachnodae NBRC 107,786T (97.82%) and Xylanimonas allomyrinae 2JSPR-7T(97.75%) with 100% completeness for the mentioned strains of Xylanimonas. The genome size of PW21T was 3.4 Mbp with a G+C content of 73.0 mol% (draft genome sequence). The average nucleotide identity (ANI) value of the draft genomes between PW21T and related species were found in between 76.99 and 78.93%. Major cellular fatty acids (> 10% of total fatty acids) of PW21Twere iso-C15:0, anteiso-C15:0, and C16:0. Phylogenetic, physiological, and biochemical characterisation identified strain PW21T (= KCTC 49,338T = JCM 33,795T = MCC 3936T) as a novel species of the genus Xylanimonas, and hence, name Xylanimonas oleitrophica sp. nov. PW21T is proposed.



中文翻译:

油养木单胞菌属 11月,从印度油藏中分离出的一种新型石油烃降解菌

从印度古吉拉特邦 Limbodara 油藏采出的水样中分离出一种新型革兰氏染色阳性、需氧、不形成孢子和杆状细菌,命名为 PW21。生长发生在 30–45 °C(最佳 37 °C)、pH 6.5–9.5(最佳 pH 8.5)和存在 0.5–5.0% (w/v) NaCl [最佳,3% (w/v) ]。基于16S rRNA基因序列的系统发育分析表明,PW21 Ť属于类放线菌,为了放线菌目,家庭Promicromonosporaceae,和属Xylanimonas,具有最高的序列相似性Isoptericola黄瓜AP38(97.95%与98.8%完整性),其次是Xylanimonas厚节虫科NBRC 107,786 T (97.82%) 和Xylanimonas allomyrinae 2JSPR-7 T (97.75%) 对上述木单胞菌菌株具有 100% 的完整性。PW21 T的基因组大小为 3.4 Mbp,G+C 含量为 73.0 mol%(基因组序列草案)。PW21 T和相关物种之间的基因组草图的平均核苷酸同一性 (ANI) 值介于 76.99 和 78.93% 之间。PW21 T 的主要细胞脂肪酸(> 10% 的总脂肪酸)是 iso-C 15:0、anteiso-C 15:0和 C 16:0。系统发育、生理和生化特征鉴定菌株 PW21 T(= KCTC 49338 Ť  = JCM 33795 Ť  = MCC 3936 Ť)作为属的一个新种Xylanimonas,因此,名称Xylanimonas oleitrophica藻。十一月 建议使用PW21 T。

更新日期:2021-01-04
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