Virus populations of ectomycorrhizal fungi remain poorly studied. In the present study, we characterized a new partitivirus isolated from the basidiomycetous, ectomycorrhizal fungus Hygrophorus penarioides, named “Hygrophorus penarioides partitivirus 1” (HpPV1). The whole genome of HpPV1, determined by merging deep sequencing and RLM-RACE approaches, comprised two dsRNA segments of 2053 bp and 2072 bp, respectively. Both dsRNA genome segments included a single open reading frame (ORF), encoding a putative RNA-dependent RNA polymerase (RdRp), and a capsid protein (CP), respectively. Based on BLASTp search, the sequences of the RdRp and CP exhibits the highest similarity (67.49% and 75.61% identity, respectively) to those of partitiviruses identified from an ascomycetous ectomycorrhizal fungus Sarcosphaera coronaria. Phylogenetic analyses performed based on the CP and RdRp sequences demonstrated that HpPV1 clusters within a clade that includes members of the genus Alphapartitivirus, belonging to the family Partitiviridae. Here, we propose that HpPV1 is a new member of the genus Alphapartitivirus. This is the first study reporting on a new partitivirus identified from the basidiomycetous, ectomycorrhizal fungus Hygrophorus penarioides.

外生菌根真菌的病毒种群仍然缺乏研究。在本研究中，我们表征了从担子菌属外生菌根真菌Hygrophorus penarioides 中分离出的一种新的局部病毒，命名为“Hygrophorus penarioides partitivirus 1”（HpPV1）。通过合并深度测序和 RLM-RACE 方法确定的 HpPV1 的全基因组分别包含两个 2053 bp 和 2072 bp 的 dsRNA 片段。两个 dsRNA 基因组片段都包含一个单一的开放阅读框 (ORF)，分别编码假定的 RNA 依赖性 RNA 聚合酶 (RdRp) 和衣壳蛋白 (CP)。基于 BLASTp 搜索，RdRp 和 CP 的序列与从子囊菌外生菌根真菌中鉴定的局部病毒的序列表现出最高的相似性（分别为 67.49% 和 75.61%）Sarcosphaera coronaria。基于 CP 和 RdRp 序列进行的系统发育分析表明，HpPV1 在一个进化枝内聚集，该进化枝包括属于Partitiviridae家族的Alphapartitivirus属的成员。在这里，我们建议HpPV1是属的一个新成员Alphapartitivirus。这是第一项关于从担子菌属外生菌根真菌Hygrophorus penarioides 中鉴定出的新型局部病毒的报告。