In the present study, a protocol for monoconidial isolation of Pyricularia oryzae from infected field samples has been illustrated. This technique is based on tapping bunch of conidia on 3% water agar medium and then transferring a single germinating conidium in fresh Potato Dextrose Agar (PDA) medium following 24 hours of incubation. This protocol allows to easily locate single conidial sites, greatly shortened overall time per isolation and remarkably lowered contamination rate during isolation. Moreover, it is more convenient and effective method for the monoconidial isolation of Pyricularia oryzae that can be exploited in less funded laboratories working with this pathogen.

中文翻译：

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一种改进的有效分离稻瘟病菌单孢菌的方案

在本研究中，已经说明了从受感染的田间样品中分离稻瘟病菌的单分生孢子菌的方案。该技术基于在 3% 水琼脂培养基上敲击一串分生孢子，然后在孵育 24 小时后将单个发芽分生孢子转移到新鲜的马铃薯葡萄糖琼脂 (PDA) 培养基中。该协议允许轻松定位单个分生孢子位点，大大缩短每次隔离的总时间并显着降低隔离期间的污染率。此外，它是一种更方便、更有效的分离稻瘟病菌的方法，可以在资金较少的实验室中利用这种病原体。