Owing to increasing concern regarding human well-being and quality of life, the demand for natural products is increased, leading to the development of a market for biological and natural products. This study aimed to investigate the depigmentation and anti-ageing properties of red seaweed (Pyropia yezoensis) extracts for skin care applications. Within the tested range of concentrations (100, 200, 400, and 800 μg mL⁻¹), P. yezoensis extracts did not exert cytotoxic effects on the three skin cell lines tested: mouse melanocytes (Melan-A), human dermal fibroblasts (1064 SK), and human dermal keratinocytes (HaCaT cells). No significant statistical difference (p = 0.05) was detected in the melanin content between cells exposed to 100 μg mL⁻¹ of arbutin and 800 μg mL⁻¹ of P. yezoensis extracts, indicating that P. yezoensis extracts had an potent inhibitory effect on melanogenesis as arbutin. There was a significant decrease in tyrosinase activity by 59.3% and 35.5% after treatment with arbutin and P. yezoensis extracts, respectively. These results indicated that P. yezoensis extracts had strong tyrosinase inhibitory properties but were not as effective as arbutin. P. yezoensis extracts also promoted collagen production by inhibiting collagen-degrading enzymes (matrix metalloproteinase (MMP)-2 and MMP-9) and promoting procollagen synthesis enzymes (tyrosinase-related protein (TRP)-1 and TRP-2). Evaluation of skin brightness and melanin content and dermatological assessment showed that P. yezoensis extracts enhanced skin brightness and reduced melanin content in 23 volunteers. Our results suggest that P. yezoensis extracts can be used as functional cosmetic agents to prevent, or remediate skin ageing and pigmentation.

由于对人类福祉和生活质量的日益关注，对天然产物的需求增加，从而导致了生物和天然产物市场的发展。这项研究旨在研究用于护肤的红海藻（Pyropia yezoensis）提取物的脱色和抗衰老特性。在所测试的浓度范围（100、200、400和800μgmL ^-1）中，yezoensis提取物未对所测试的三种皮肤细胞系产生细胞毒性作用：小鼠黑素细胞（Melan-A），人皮肤成纤维细胞（ 1064 SK）和人类皮肤角质形成细胞（HaCaT细胞）。差异无统计学意义（p 在暴露于100μgmL ^-1的熊果素和800μgmL ^-1的紫苏提取物之间的细胞中，黑色素含量被检测到= 0.05），这表明紫苏提取物像熊果苷一样对黑素生成具有有效的抑制作用。用熊果苷和野牛假单胞菌提取物处理后，酪氨酸酶活性分别显着降低了59.3％和35.5％。这些结果表明，耶氏疟原虫提取物具有强的酪氨酸酶抑制特性，但不如熊果苷有效。野zo提取物还通过抑制胶原降解酶（基质金属蛋白酶（MMP）-2和MMP-9）和促进胶原蛋白合成酶（酪氨酸酶相关蛋白（TRP）-1和TRP-2）来促进胶原蛋白的产生。皮肤亮度和黑色素含量的评估以及皮肤病学评估表明，紫叶番石榴提取物在23名志愿者中增强了皮肤的亮度，减少了黑色素的含量。我们的研究结果表明，黑斑病杆菌提取物可用作功能性美容剂，以预防或补救皮肤衰老和色素沉着。