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Pigment content analysis in two HAB forming dinoflagellate species during the growth period
Journal of Applied Phycology ( IF 3.3 ) Pub Date : 2021-01-04 , DOI: 10.1007/s10811-020-02331-w
Somayeh Zahedi Dizaji , Gilan Attaran Fariman , Mir Mahdi Zahedi

Dinoflagellates form a large group of microalgae that have different pigments and the pigment content can vary depending on environmental conditions such as temperature, salinity, and their growth phase. They have the potential to cause red tides in coastal waters, so it is important to identify their pigments. In this study, two bloom forming dinoflagellate species, Scrippsiella trochoidea and Gyrodinium instriatum from the Oman Sea, were studied for their pigment content by HPLC every 5 days over a 30-day growth period. In both species, 10 pigment types were identified: unknown carotenoids, chlorophyll (chl) c2, peridinin, peridinin-like, diadinochrome, diadinoxanthin, dinoxanthin, diatoxanthin, chl a, and β,β-carotene. Although both species have the same pigment types, they vary in concentration in different days of growth. The highest cell density in S. trochoidea species was on day 15 of the growth period and the predominant pigments were chl c2, diadinoxanthin, and chl a. Peridinin, peridinin-like, diadinochrome, and β,β-carotene were predominant pigments on day 10, before the cell reached its maximum density. In G. instriatum, the highest cell density during the growth period was in day 10. The highest levels of unknown carotenoids, chl c2, diadinoxanthin, dinoxanthin, peridinin, peridinin-like, diadinochrome, and β,β-carotene pigments were measured on day 25 of growth. The type of chloroplast in both species was the peridinin type. In interpreting the pigment concentration and type, the age of the species should be considered.



中文翻译:

生长期形成两个鞭毛藻的两个HAB色素含量分析

藻鞭毛藻形成一大群微藻,它们具有不同的色素,色素含量可根据环境条件(例如温度,盐度及其生长阶段)而变化。它们有可能在沿海水域引起赤潮,因此识别其色素很重要。在这项研究中,在30天的生长期中,每隔5天用HPLC对来自阿曼海的两种形成花白鞭藻的物种鞭毛印度回旋藻进行了色素含量的研究。在这两个物种,10种颜料类型被确定:未知类胡萝卜素,叶绿素(叶绿素)c ^ 2,多甲藻素,多甲藻素类,diadinochrome,diadinoxanthin,dinoxanthin,diatoxanthin,叶绿素一个和β,β-胡萝卜素。尽管两种物种具有相同的色素类型,但它们在不同的生长日中浓度会有所不同。trochoidea物种的最高细胞密度是在生长期的第15天,主要色素是chl c 2,二恶黄质和chl a。在细胞达到最大密度之前的第10天,皮兰定素,类皮素,类二色染料和β,β-胡萝卜素是主要的色素。在G. instriatum中,生长期间的最高细胞密度是在第10天。未知类胡萝卜素的最高水平chl c 2在生长的第25天,测定了二恶英,二恶英,二恶黄素,peridinin,类peridinin,二色铬和β,β-胡萝卜素的色素。两种物种中的叶绿体类型均为peridinin类型。在解释颜料浓度和类型时,应考虑物种的年龄。

更新日期:2021-01-04
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