Abstract

Several fungal endophytes were isolated and screened for their ability to biosynthesize a variety of nanoparticles (NPs), as a potentially simple and eco-friendly method with low cost. Among these fungi, a promising isolate named ORG-1 was found able to synthesize five different NPs types: Co₃O₄NPs, CuONPs, Fe₃O₄NPs, NiONPs, and ZnONPs. The ORG-1 strain was identified as Aspergillus terreus according to the morphological and molecular studies. Synthesis of these NPs was initially monitored by UV–Vis spectroscopy and further characterized by Fourier transform infrared spectroscopy. X-ray diffraction patterns revealed their crystalline structure. Dynamic light scattering analysis was applied to study the particle size distribution and stability. Transmission electron microscope studies indicated the morphology of the synthesized NPs. Additionally, the biological activities of the in vitro antioxidant and antimicrobial potentials were evaluated. Co₃O₄NPs, CuONPs, Fe₃O₄NPs, NiONPs, and ZnONPs showed promising antioxidant activity with 50% inhibitory concentrations of 85.44, 96.74, 102.41, 87.41, and 108.67 μg mL⁻¹, respectively. The synthesized NPs exhibited potent antimicrobial activities against several plant and human pathogens. To our knowledge, this is the first report on the use of one microbial strain for the synthesis of a variety of NPs. This study suggests endophytic fungi as new and alternate platforms with an exceptional potentiality for the synthesis of NPs with promising activities.

Key points

• Discovery of a promising endophytic fungus for synthesis of five different types of NPs.

• Mycosynthesis and characterization of all the synthesized NPs were investigated.

• The synthesized NPs showed promising antioxidant and antimicrobial activities.

中文翻译：

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内生曲霉新型合成Co 3 O 4，CuO，Fe 3 O 4，NiO和ZnO纳米颗粒及其抗氧化性和抗菌活性的评价。

摘要

分离并筛选了几种真菌内生菌，以生物合成多种纳米颗粒（NP）的能力，这是一种潜在的简单且环保的低成本方法。在这些真菌中，发现了一种有前途的分离株ORG-1，能够合成五种不同的NP类型：Co ₃ O ₄ NP，CuONP，Fe ₃ O ₄ NP，NiONP和ZnONP。ORG-1菌株被鉴定为土曲霉根据形态和分子研究。这些NP的合成最初是通过UV-Vis光谱监测的，并通过Fourier变换红外光谱进一步表征的。X射线衍射图表明它们的晶体结构。动态光散射分析用于研究粒度分布和稳定性。透射电子显微镜研究表明了合成的NP的形态。另外，评估了体外抗氧化剂的生物活性和抗菌潜力。Co ₃ O ₄ NPs，CuONPs，Fe ₃ O ₄ NPs，NiONPs和ZnONPs表现出良好的抗氧化活性，抑制浓度为50.44、96.74、102.41、87.41和108.67μg/ mL，浓度为50％^-1。合成的纳米粒对多种植物和人类病原体均表现出强大的抗菌活性。据我们所知，这是关于使用一种微生物菌株合成各种NP的首次报道。这项研究表明内生真菌是新的和可替代的平台，具有合成具有前途活性的NP的巨大潜力。

关键点

•发现一种有前途的内生真菌，可合成五种不同类型的NP。

•研究了所有合成NP的真菌合成和表征。

•合成的NPs显示出有希望的抗氧化和抗菌活性。